BMRB Entry 6476

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PDB ID: 1yqa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6476
MolProbity Validation Chart

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Title:
Full 1H, 13C and 15N Chemical Shift Assignments for the GII loop mutant domain (GII-L) of the yeast linker histone, Hho1p
Deposition date:
2005-02-01
Original release date:
2005-09-22
Authors:
Sanderson, Andrew; Stott, Katherine; Stevens, Timothy; Thomas, Jean
Citation:

Citation: Sanderson, Andrew; Stott, Katherine; Stevens, Timothy; Thomas, Jean. "Engineering the structural stability and functional properties of the GI domain into the intrinsically unfolded GII domain of the yeast linker histone Hho1p."  J. Mol. Biol. 349, 608-620 (2005).
PubMed: 15878177

Assembly members:

Assembly members:
GII-L, polymer, 87 residues, 9537 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GII-L: KASSPSSLTYKEMILKSMPQ LNDGKGSSRIVLKKYVKDTY PIVGSASNFDYLFNSAIKKC VENGELVQPKGPSGIIKLNK KKVKLST

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts88
1H chemical shifts636

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1GII-L1

Entities:

Entity 1, GII-L 87 residues - 9537 Da.

1   LYSALASERSERPROSERSERLEUTHRTYR
2   LYSGLUMETILELEULYSSERMETPROGLN
3   LEUASNASPGLYLYSGLYSERSERARGILE
4   VALLEULYSLYSTYRVALLYSASPTHRTYR
5   PROILEVALGLYSERALASERASNPHEASP
6   TYRLEUPHEASNSERALAILELYSLYSCYS
7   VALGLUASNGLYGLULEUVALGLNPROLYS
8   GLYPROSERGLYILEILELYSLEUASNLYS
9   LYSLYSVALLYSLEUSERTHR

Related Database Links:

PDB 1YQA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
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