BMRB Entry 5933

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PDB ID: 1x9x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5933
MolProbity Validation Chart

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Title:
Sequence-specific backbone and sidechain resonance assignments of the Ste50 binding domain of the MAPKKK Ste11
Deposition date:
2003-09-05
Original release date:
2005-03-01
Authors:
Bhattacharjya, Surajit; Xu, Ping; Shaykhutdinov, Rustem; Gingras, Richard; Ni, Feng
Citation:

Citation: Bhattacharjya, Surajit; Xu, Ping; Gingras, R.; Shaykhutdinov, R.; Wu, C.; Whiteway, M.; Ni, Feng. "Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex"  J. Mol. Biol. 344, 1071-1087 (2004).
PubMed: 15544813

Assembly members:

Assembly members:
SAM domain of Ste11, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Budding yeast   Taxonomy ID: 4932   Superkingdom: Eucaryote   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAM domain of Ste11: DEKTNDLPFVQLFLEEIGCT QYLDSFIQCNLVTEEEIKYL DKDILIALGVNKIGDRLKIL RKSKSFQR

Data sets:
Data typeCount
1H chemical shifts474
13C chemical shifts299
15N chemical shifts72

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ste11 SAM, chain 11
2Ste11 SAM, chain 21

Entities:

Entity 1, Ste11 SAM, chain 1 68 residues - Formula weight is not available

1   ASPGLULYSTHRASNASPLEUPROPHEVAL
2   GLNLEUPHELEUGLUGLUILEGLYCYSTHR
3   GLNTYRLEUASPSERPHEILEGLNCYSASN
4   LEUVALTHRGLUGLUGLUILELYSTYRLEU
5   ASPLYSASPILELEUILEALALEUGLYVAL
6   ASNLYSILEGLYASPARGLEULYSILELEU
7   ARGLYSSERLYSSERPHEGLNARG

Related Database Links:

PDB 1OW5 1X9X
DBJ GAA25223
EMBL CAA37522 CAY81586
GB AAB67571 AHY78717 EDN59268 EDZ70440 EEU06456
REF NP_013466
SP A7A1P0 B5VNQ3 P23561
TPG DAA09666
AlphaFold A7A1P0 B5VNQ3 P23561

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks