BMRB Entry 5738

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PDB ID: 1npq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5738
MolProbity Validation Chart

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Title:
The NMR Structure of a Bifunctional Rhodamine Labeled N-domain of Troponin C in Complex with the Regulatory 'Switch' Peptide from Troponin I
Deposition date:
2003-03-14
Original release date:
2003-08-07
Authors:
Mercier, P.; Ferguson, R.; Irving, M.; Corrie, J.; Trentham, D.; Sykes, B.
Citation:

Citation: Mercier, P.; Ferguson, R.; Irving, M.; Corrie, J.; Trentham, D.; Sykes, B.. "NMR Structure of a Bifunctional Rhodamine Labeled N-domain of Troponin C Complexed with the Regulatory 'Switch' Peptide from Troponin I: Implications for in situ Fluorescence Studies in Muscle Fibers "  Biochemistry 42, 4333-4348 (2003).
PubMed: 12693929

Assembly members:

Assembly members:
Troponin C, polymer, 90 residues, Formula weight is not available
Troponin I, polymer, 17 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET3A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Troponin C: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKCELDA IICEVDEDGSGTIDFEEFLV MMVRQMKEDA
Troponin I: RMSADAMLRALLGSKHK

Data sets:
Data typeCount
1H chemical shifts629
13C chemical shifts263
15N chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Troponin C1
2Troponin I2
3CALCIUM ION(II) 13
4CALCIUM ION(II) 23

Entities:

Entity 1, Troponin C 90 residues - Formula weight is not available

1   ALASERMETTHRASPGLNGLNALAGLUALA
2   ARGALAPHELEUSERGLUGLUMETILEALA
3   GLUPHELYSALAALAPHEASPMETPHEASP
4   ALAASPGLYGLYGLYASPILESERTHRLYS
5   GLULEUGLYTHRVALMETARGMETLEUGLY
6   GLNASNPROTHRLYSCYSGLULEUASPALA
7   ILEILECYSGLUVALASPGLUASPGLYSER
8   GLYTHRILEASPPHEGLUGLUPHELEUVAL
9   METMETVALARGGLNMETLYSGLUASPALA

Entity 2, Troponin I 17 residues - Formula weight is not available

1   ARGMETSERALAASPALAMETLEUARGALA
2   LEULEUGLYSERLYSHISLYS

Entity 3, CALCIUM ION(II) 1 - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 16414
PDB 1NPQ 1YTZ 1YV0 2W49 2W4U
EMBL CAA27447 CAA27447
GB AAA61952 AAB00122 AAH44282 AAH84508 AAL86905
REF NP_001011110 NP_001079556 NP_990748 XP_001518819 XP_002199061 XP_001518819 NP_990748 NP_001079556 NP_001011110
SP P68246 P68247
SWISS-PROT P68247 P68246
GenBank AAL86905 AAH84508 AAH44282 AAB00122 AAA61952
AlphaFold P68246 P68247

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