BMRB Entry 5622

Title:
Structural and Functional Characterization of a Thioredoxin-like Protein from methanobacterium thermoautotrophicum (Delta H)
Deposition date:
2002-12-11
Original release date:
2003-08-08
Authors:
Amegbey, Godwin; Monzavi, Hassan; Habibi-Nazad, Bahram; Bhattacharyya, Sudeepa; Wishart, David
Citation:

Citation: Amegbey, Godwin; Monzavi, Hassan; Habibi-Nazhad, Bahram; Bhattacharyya, Sudeepa; Wishart, David. "Structural and Functional Characterization of a Thioredoxin-like Protein (Mt0807) from Methanobacterium thermoautotrophicum"  Biochemistry 42, 8001-8010 (2003).
PubMed: 12834352

Assembly members:

Assembly members:
Thioredoxin from M. thermoautotrophicum, polymer, 85 residues, 9481 Da.

Natural source:

Natural source:   Common Name: Methanothermobacter thermoautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Thioredoxin from M. thermoautotrophicum: MVVNIEVFTSPTCPYCPMAI EVVDEAKKEFGDKIDVEKID IMVDREKAIEYGLMAVPAIA INGVVRFVGAPSREELFEAI NDEME

Data sets:
Data typeCount
1H chemical shifts582
13C chemical shifts241
15N chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mt08071

Entities:

Entity 1, Mt0807 85 residues - 9481 Da.

1   METVALVALASNILEGLUVALPHETHRSER
2   PROTHRCYSPROTYRCYSPROMETALAILE
3   GLUVALVALASPGLUALALYSLYSGLUPHE
4   GLYASPLYSILEASPVALGLULYSILEASP
5   ILEMETVALASPARGGLULYSALAILEGLU
6   TYRGLYLEUMETALAVALPROALAILEALA
7   ILEASNGLYVALVALARGPHEVALGLYALA
8   PROSERARGGLUGLULEUPHEGLUALAILE
9   ASNASPGLUMETGLU

Samples:

sample_1: Thioredoxin from M. thermoautotrophicum, [U-13C; U-15N], 1 mM; NaH2PO4 50 mM; NaCl 100 mM

Sample_conditions_1: pH: 6.0; temperature: 298 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-NOESYsample_1not availableSample_conditions_1
2D 1H-TOCSYsample_1not availableSample_conditions_1
1H-15N HSQCsample_1not availableSample_conditions_1
3D 1H-15N NOESY HSQCsample_1not availableSample_conditions_1
3D 1H-15N TOCSY-HSQCsample_1not availableSample_conditions_1
HNCOsample_1not availableSample_conditions_1
HNCACBsample_1not availableSample_conditions_1

Software:

VNMR v6.1C -

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

PDB
DBJ BAM69972
GB AAB85307
REF NP_275946 WP_010876442
SP O26898
AlphaFold O26898

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks