BMRB Entry 5529

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PDB ID: 1m9g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5529
MolProbity Validation Chart

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Title:
The structure of G16A MNEI monellin supports a new model of interaction of sweet proteins with the T1R2-T1R3 receptor
Deposition date:
2002-09-13
Original release date:
2003-07-07
Authors:
Spadaccini, R.; Trabucco, F.; Saviano, G.; Picone, D.; Crescenzi, O.; Tancredi, T.; Temussi, P.
Citation:

Citation: Spadaccini, R.; Trabucco, F.; Saviano, G.; Picone, D.; Crescenzi, O.; Tancredi, T.; Temussi, P.. "The Mechanism of Interaction of Sweet Proteins with the T1R2-T1R3 Receptor: Evidence from the Solution Structure of G16A-MNEI"  J. Mol. Biol. 328, 683-692 (2003).
PubMed: 12706725

Assembly members:

Assembly members:
monellin, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: serendipity berry   Taxonomy ID: 3457   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Dioscoreophyllum cumminsii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET22B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
monellin: MGEWEIIDIGPFTQNLAKFA VDEENKIGQYGRLTFNKVIR PCMKKTIYENEGFREIKGYE YQLYVYASDKLFRADISEDY KTRGRKLLRFNGPVPPP

Data sets:
Data typeCount
15N chemical shifts104
1H chemical shifts587

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Monellin chains B and A1

Entities:

Entity 1, Monellin chains B and A 97 residues - Formula weight is not available

1   METGLYGLUTRPGLUILEILEASPILEGLY
2   PROPHETHRGLNASNLEUALALYSPHEALA
3   VALASPGLUGLUASNLYSILEGLYGLNTYR
4   GLYARGLEUTHRPHEASNLYSVALILEARG
5   PROCYSMETLYSLYSTHRILETYRGLUASN
6   GLUGLYPHEARGGLUILELYSGLYTYRGLU
7   TYRGLNLEUTYRVALTYRALASERASPLYS
8   LEUPHEARGALAASPILESERGLUASPTYR
9   LYSTHRARGGLYARGLYSLEULEUARGPHE
10   ASNGLYPROVALPROPROPRO

Related Database Links:

BMRB 4638
PDB 1FA3 1IV7 1M9G 2O9U 3MON 3PXM 3PYJ 3Q2P 4MON
GB AFF58925
PRF 761801B

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