BMRB Entry 4934

Title:
Structure and functionality of a designed p53 dimer
Deposition date:
2000-12-21
Original release date:
2001-01-23
Authors:
Davison, T.; Nie, X.; Ma, W.; Lin, Y.; Kay, C.; Benchimol, S.; Arrowsmith, Cheryl
Citation:

Citation: Davison, T.; Nie, X.; Ma, W.; Lin, Y.; Kay, C.; Benchimol, S.; Arrowsmith, Cheryl. "Structure and functionality of a designed p53 dimer"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
p53, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p53: DGEYFTLQIRGRERFEQFRE RNEALELKDAQAG

Data sets:
Data typeCount
13C chemical shifts95
15N chemical shifts37
1H chemical shifts228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p53 subunit 11
2p53 subunit 21

Entities:

Entity 1, p53 subunit 1 33 residues - Formula weight is not available

1   ASPGLYGLUTYRPHETHRLEUGLNILEARG
2   GLYARGGLUARGPHEGLUGLNPHEARGGLU
3   ARGASNGLUALALEUGLULEULYSASPALA
4   GLNALAGLY

Samples:

sample_1: p53, [U-15N; U-13C], 1.85 – 2.1 mM; D2O 10%

Ex-cond_1: ionic strength: 0.125 M; pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
(HB)CBCA(CO)NNHnot availablenot availablenot available
N15-NOESY-HSQCnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCC-TOCSYnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
15N TOCSY HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Unity Plus 500 MHz
  • Varian Unity Plus 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks