BMRB Entry 4414
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4414
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Babu, Charles; Volkman, Brian; Bullerjahn, George. "NMR Solution Structure of Plastocyanin from the Photosynthetic Prokaryote,
Prochlorothrix hollandica" Biochemistry 38, 4988-4995 (1999).
Assembly members:
plastocyanin, polymer, 97 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.
Natural source: Common Name: Prochlorothrix hollandica Taxonomy ID: 1223 Superkingdom: Eubacteria Kingdom: not available Genus/species: Prochlorothrix hollandica
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSCREEN
Entity Sequences (FASTA):
plastocyanin: ASVQIKMGTDKYAPLYEPKA
LSISAGDTVEFVMNKVGPHN
VIFDKVPAGESAPALSNTKL
AIAPGSFYSVTLGTPGTYSF
YCTPHRGAGMVGTITVE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 666 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PC | 1 |
| 2 | Cu | 2 |
Entities:
Entity 1, PC 97 residues - Formula weight is not available
| 1 | ALA | SER | VAL | GLN | ILE | LYS | MET | GLY | THR | ASP | ||||
| 2 | LYS | TYR | ALA | PRO | LEU | TYR | GLU | PRO | LYS | ALA | ||||
| 3 | LEU | SER | ILE | SER | ALA | GLY | ASP | THR | VAL | GLU | ||||
| 4 | PHE | VAL | MET | ASN | LYS | VAL | GLY | PRO | HIS | ASN | ||||
| 5 | VAL | ILE | PHE | ASP | LYS | VAL | PRO | ALA | GLY | GLU | ||||
| 6 | SER | ALA | PRO | ALA | LEU | SER | ASN | THR | LYS | LEU | ||||
| 7 | ALA | ILE | ALA | PRO | GLY | SER | PHE | TYR | SER | VAL | ||||
| 8 | THR | LEU | GLY | THR | PRO | GLY | THR | TYR | SER | PHE | ||||
| 9 | TYR | CYS | THR | PRO | HIS | ARG | GLY | ALA | GLY | MET | ||||
| 10 | VAL | GLY | THR | ILE | THR | VAL | GLU |
Entity 2, Cu
Samples:
sample_1: plastocyanin 3 mM; Potassium Phosphate 20 mM
sample_cond_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | not available | sample_cond_1 |
| TOCSY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
Software:
FELIX v97 -
NMR spectrometers:
- Bruker DMX 750 MHz