BMRB Entry 4141

Title:
vnd/NK-2 Homeodomain DNA Complex Protein 1H, 13C, and 15N Chemical Shifts and HNHA Coupling Constant
Deposition date:
1998-05-11
Original release date:
2001-03-07
Authors:
Gruschus, James; Tsao, Desiree; Wang, Lan-Hsiang; Nirenberg, Marshall; Ferretti, James
Citation:

Citation: Gruschus, James; Tsao, Desiree; Wang, Lan-Hsiang; Nirenberg, Marshall; Ferretti, James. "The three-dimensional structure of the vnd/NK-2 homeodomain-DNA complex by NMR spectroscopy"  J. Mol. Biol. 289, 529-545 (1999).
PubMed: 10356327

Assembly members:

Assembly members:
vnd/NK-2 homeodomain, polymer, 77 residues, Formula weight is not available
(+)5'TGTGTCAAGTGGCTGT, polymer, 16 residues, Formula weight is not available
(-)5'ACAGCCACTTGACACAA, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
vnd/NK-2 homeodomain: ASDGLPNKKRKRRVLFTKAQ TYELERRFRQQRYLSAPERE HLASLIRLTPTQVKIWFQNH RYKTKRAQNEKGYEGHP
(+)5'TGTGTCAAGTGGCTGT: TGTGTCAAGTGGCTGT
(-)5'ACAGCCACTTGACACAA: ACAGCCACTTGACACAA

Data typeCount
13C chemical shifts291
15N chemical shifts100
1H chemical shifts904
31P chemical shifts18
coupling constants53

Additional metadata:

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  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1vnd/NK-2 homeodomain1
2DNA duplex (+) strand2
3DNA duplex (-) strand3

Entities:

Entity 1, vnd/NK-2 homeodomain 77 residues - Formula weight is not available

1   ALASERASPGLYLEUPROASNLYSLYSARG
2   LYSARGARGVALLEUPHETHRLYSALAGLN
3   THRTYRGLULEUGLUARGARGPHEARGGLN
4   GLNARGTYRLEUSERALAPROGLUARGGLU
5   HISLEUALASERLEUILEARGLEUTHRPRO
6   THRGLNVALLYSILETRPPHEGLNASNHIS
7   ARGTYRLYSTHRLYSARGALAGLNASNGLU
8   LYSGLYTYRGLUGLYHISPRO

Entity 2, DNA duplex (+) strand 16 residues - Formula weight is not available

1   DTDGDTDGDTDCDADADGDT
2   DGDGDCDTDGDT

Entity 3, DNA duplex (-) strand 16 residues - Formula weight is not available

1   DADCDADGDCDCDADCDTDT
2   DGDADCDADCDADA

Related Database Links:

BMRB 4368
PDB 1NK2 1NK3 1QRY 1VND
EMBL CAA21410 CAA60619
GB AAA28617 AAB34960 AAF45521 ABI30961 ADJ13174
REF NP_001036253 NP_476786 XP_001354499 XP_001982405 XP_001992446
SP P22808
AlphaFold P22808

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks