BMRB Entry 34258

Assembly:

Entities:

Entity 1, entity_1 41 residues - 4528.011 Da.

Entity 2, entity_2 5 residues - 1602.992 Da.

Entity 3, entity_3 - Zn - 65.409 Da.

Samples:

sample_1: FUS-ZnF, [U-99% 13C; U-99% 15N], 0.8 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 1.0 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_2: FUS-ZnF, [U-99% 13C; U-99% 15N], 0.8 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 1.0 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_3: FUS-ZnF, [U-99% 15N], 1.0 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 0.9 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_4: FUS-ZnF, [U-99% 13C; U-99% 15N], 1.0 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 0.9 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 Pa; temperature: 303 K

sample_conditions_2: ionic strength: 20 mM; pH: 6.5; pressure: 1 Pa; temperature: 278 K

Experiments:

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CANDID, Herrmann, Guntert and Wuthrich - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

• Bruker Avance 900 MHz
• Bruker AvanceIII 700 MHz
• Bruker AvanceIII 600 MHz