BMRB Entry 30314
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PDB ID: 5wbt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30314
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Glidden, Michael; Yang, Yanwu; Smith, Nicholas; Phillips, Nelson; Carr, Kelley; Wickramasinghe, Nalinda; Ismail-Beigi, Faramarz; Lawrence, Michael; Smith, Brian; Weiss, Michael. "Solution structure of an ultra-stable single-chain insulin analog connects protein dynamics to a novel mechanism of receptor binding" J. Biol. Chem. 293, 69-88 (2018).
PubMed: 29114034
Assembly members:
Insulin, polymer, 57 residues, 6515.308 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia
Entity Sequences (FASTA):
Insulin: FVNQHLCGSHLVEALYLVCG
ERGFFYTDPTEEGPRRGIVE
QCCHSICSLEQLENYCN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 205 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 299 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 57 residues - 6515.308 Da.
| 1 | PHE | VAL | ASN | GLN | HIS | LEU | CYS | GLY | SER | HIS | ||||
| 2 | LEU | VAL | GLU | ALA | LEU | TYR | LEU | VAL | CYS | GLY | ||||
| 3 | GLU | ARG | GLY | PHE | PHE | TYR | THR | ASP | PRO | THR | ||||
| 4 | GLU | GLU | GLY | PRO | ARG | ARG | GLY | ILE | VAL | GLU | ||||
| 5 | GLN | CYS | CYS | HIS | SER | ILE | CYS | SER | LEU | GLU | ||||
| 6 | GLN | LEU | GLU | ASN | TYR | CYS | ASN |
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