BMRB Entry 30220

Click here to enlarge.

PDB ID: 5ub0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30220
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution NMR Structure of NERD-C, a natively folded tetramutant of the B1 domain of streptococcal protein G (GB1)
Deposition date:
2016-12-20
Original release date:
2017-08-16
Authors:
Damry, A.; Davey, J.; Goto, N.; Chica, R.
Citation:

Citation: Davey, J.; Damry, A.; Goto, N.; Chica, R.. "Rational design of proteins that exchange on functional timescales."  Nat. Chem. Biol. 13, 1280-1285 (2017).
PubMed: 29058725

Assembly members:

Assembly members:
entity_1, polymer, 56 residues, 6165.795 Da.

Natural source:

Natural source:   Common Name: Streptococcus sp. GX7805   Taxonomy ID: 1325   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus Streptococcus sp. GX7805

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTFKLIINGKTLKGETTTEA VDAATAEKVLKQYANDNGID GEWTYDDATKTFTVTE

Data typeCount
13C chemical shifts387
15N chemical shifts61
1H chemical shifts635

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 56 residues - 6165.795 Da.

1   METTHRPHELYSLEUILEILEASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALLEU
4   LYSGLNTYRALAASNASPASNGLYILEASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks