BMRB Entry 27741
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PDB ID: 2ky9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27741
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Rosario-Cruz, Zuelay; Eletsky, Alexander; Daigham, Nourhan; Al-Tameemi, Hassan; Swapna, G; Kahn, Peter; Szyperski, Thomas; Montelione, Gaetano; Boyd, Jeffrey. "The" J. Biol. Chem. 294, 4027-4044 (2019).
PubMed: 30655293
Assembly members:
bsCopL, polymer, 128 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15
Entity Sequences (FASTA):
bsCopL: SHMKVGSQVIINTSHMKGMK
GAEATVTGAYDTTAYVVSYT
PTNGGQRVDHHKWVIQEEIK
DAGDKTLQPGDQVILEASHM
KGMKGATAEIDSAEKTTVYM
VDYTSTTSGEKVKNHKWVTE
DELSAKLE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bsCopL | 1 |
Entities:
Entity 1, bsCopL 128 residues - Formula weight is not available
Residues SHM result from cleavage of the N-terminal His-tag by a TEV protease. Residues K4 of the above sequence corresponds to residues K83 of the protein.
| 1 | SER | HIS | MET | LYS | VAL | GLY | SER | GLN | VAL | ILE | ||||
| 2 | ILE | ASN | THR | SER | HIS | MET | LYS | GLY | MET | LYS | ||||
| 3 | GLY | ALA | GLU | ALA | THR | VAL | THR | GLY | ALA | TYR | ||||
| 4 | ASP | THR | THR | ALA | TYR | VAL | VAL | SER | TYR | THR | ||||
| 5 | PRO | THR | ASN | GLY | GLY | GLN | ARG | VAL | ASP | HIS | ||||
| 6 | HIS | LYS | TRP | VAL | ILE | GLN | GLU | GLU | ILE | LYS | ||||
| 7 | ASP | ALA | GLY | ASP | LYS | THR | LEU | GLN | PRO | GLY | ||||
| 8 | ASP | GLN | VAL | ILE | LEU | GLU | ALA | SER | HIS | MET | ||||
| 9 | LYS | GLY | MET | LYS | GLY | ALA | THR | ALA | GLU | ILE | ||||
| 10 | ASP | SER | ALA | GLU | LYS | THR | THR | VAL | TYR | MET | ||||
| 11 | VAL | ASP | TYR | THR | SER | THR | THR | SER | GLY | GLU | ||||
| 12 | LYS | VAL | LYS | ASN | HIS | LYS | TRP | VAL | THR | GLU | ||||
| 13 | ASP | GLU | LEU | SER | ALA | LYS | LEU | GLU |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
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