BMRB Entry 27349

Title:
1H, 13C, 15N chemical shift assignments of initiation factor 1 from Clostridium difficile
Deposition date:
2017-12-26
Original release date:
2019-01-10
Authors:
Zhang, Yonghong; Aguilar, Faith
Citation:

Citation: Aguilar, Faith; Banaei, Niaz; Zhang, Yonghong. "1H, 13C, 15N chemical shift assignments of initiation factor 1 from Clostridium difficile"  Biomol. NMR Assignments 13, 91-95 (2019).
PubMed: 30370502

Assembly members:

Assembly members:
C_difficile_IF1, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium difficile   Taxonomy ID: 1496   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium difficile

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts74
1H chemical shifts483

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C. difficile IF11

Entities:

Entity 1, C. difficile IF1 83 residues - Formula weight is not available

Residues- MAS at the very beginning and Residues-LEHHHHHH at the end represent non-native residues because of subcloning and affinity tag.

1   METALASERMETALALYSLYSASPVALILE
2   GLULEUGLUGLYTHRVALSERGLUALALEU
3   PROASNALAMETPHELYSVALLYSLEUGLU
4   ASNGLYHISGLUILELEUCYSHISILESER
5   GLYLYSLEUARGMETASNPHEILEARGILE
6   LEUGLUGLYASPLYSVALASNVALGLULEU
7   SERPROTYRASPLEUTHRARGGLYARGILE
8   THRTRPARGLYSLYSLEUGLUHISHISHIS
9   HISHISHIS

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