BMRB Entry 27271

Name: Solution NMR structure of BCoR in complex with AF9 (BCoR-AF9)
Published: 2018-10-01

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PDB ID: 6b7g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27271
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of BCoR in complex with AF9 (BCoR-AF9)

Deposition date:

2017-10-03

Original release date:

2018-10-01

Authors:

Schmidt, Charles; Kuntimaddi, Aravinda; Leach, Benjamin; Bushweller, John

Citation:

Citation: Schmidt, Charles; Achille, Nicholas; Kuntimaddi, Aravinda; Boulton, Adam; Leach, Benjamin; Zeleznik-Le, Nancy; Bushweller, John. "Direct Recruitment of BCoR, but not CBX8, is Required for MLL-AF9 Leukemia" .

Assembly members:

Assembly members:
AF9, polymer, 70 residues, Formula weight is not available
BCoR, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET Duet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AF9: MDKAYLDELVELHRRLMTLR ERHILQQIVNLIEETGHFHI TNTTFDFDLCSLDKTTVRKL QSYLETSGTS
BCoR: GTTNSSSNHLEDPHYSELTN LKVCIELTGLHPKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	448
15N chemical shifts	104
1H chemical shifts	691

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AF9	1
2	BCoR	2

Entities:

Entity 1, AF9 70 residues - Formula weight is not available

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER

Entity 2, BCoR 34 residues - Formula weight is not available

1

GLY

THR

ASN

SER

ASN

HIS

LEU

2

GLU

ASP

PRO

HIS

TYR

SER

GLU

LEU

THR

ASN

3

LEU

LYS

VAL

CYS

ILE

GLU

LEU

THR

GLY

LEU

4

HIS

PRO

LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	BCoR-AF9	isotropic	sample_conditions
2D 1H-13C HSQC	BCoR-AF9	isotropic	sample_conditions
3D HNCO	BCoR-AF9	isotropic	sample_conditions
3D HNHA	BCoR-AF9	isotropic	sample_conditions
3D HNCACB	BCoR-AF9	isotropic	sample_conditions
3D CBCA(CO)NH	BCoR-AF9	isotropic	sample_conditions
3D HCCH-TOCSY	BCoR-AF9	isotropic	sample_conditions
3D C(CO)NH	BCoR-AF9	isotropic	sample_conditions
3D 1H-13C NOESY aromatic	BCoR-AF9	isotropic	sample_conditions
3D 1H-13C NOESY aliphatic	BCoR-AF9	isotropic	sample_conditions
3D 1H-15N NOESY	BCoR-AF9	isotropic	sample_conditions

BMRB Entry 27271

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER