BMRB Entry 26691

Title:
Chemical shift assignment of yeast Bcd1 protein zinc finger
Deposition date:
2015-10-22
Original release date:
2016-05-23
Authors:
Bragantini, Benoit; Quinternet, Marc; Manival, Xavier
Citation:

Citation: Bragantini, Benoit; Quinternet, Marc; Manival, Xavier; Charpentier, Bruno; Tiotiu, Decebal; Rothe, Benjamin; Saliou, Jean-Michel; Cianferani, Sarah. "Functional and structural insights into the zinc-finger HIT protein family involved in box C/D snoRNP biogenesis"  J. Mol. Biol. 428, 2488-2506 (2016).
PubMed: 27139642

Assembly members:

Assembly members:
ZnF-Bcd1, polymer, 48 residues, 5341.2 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pnEA-3cH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ZnF-Bcd1: GPHMAVLCGVCGIKEFKYKC PRCLVQTCSLECSKKHKTRD NCSGQTHD

Data sets:
Data typeCount
13C chemical shifts369
15N chemical shifts84
1H chemical shifts583

Additional metadata:

  • Assembly
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ZnF-Bcd11
2zinc ion, 12
3zinc ion, 22

Entities:

Entity 1, ZnF-Bcd1 48 residues - 5341.2 Da.

Residues 1-3 represent non-native residues from affinity tag

1   GLYPROHISMETALAVALLEUCYSGLYVAL
2   CYSGLYILELYSGLUPHELYSTYRLYSCYS
3   PROARGCYSLEUVALGLNTHRCYSSERLEU
4   GLUCYSSERLYSLYSHISLYSTHRARGASP
5   ASNCYSSERGLYGLNTHRHISASP

Entity 2, zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks