BMRB Entry 26053

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PDB ID: 2nda
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26053
MolProbity Validation Chart

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Title:
Solution structure of MapZ extracellular domain second subdomain
Deposition date:
2016-05-11
Original release date:
2016-06-27
Authors:
Jean, Nicolas; Manuse, Sylvie; Guinot, Megane; Bougault, Catherine; Grangeasse, Christophe; Simorre, Jean-Pierre
Citation:

Citation: Manuse, Sylvie; Jean, Nicolas; Guinot, Megane; Lavergne, Jean-Pierre; Laguri, Cedric; Bougault, Catherine; Van Nieuwenhze, Michael; Grangeasse, Christophe; Simorre, Jean-Pierre. "Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ"  Nat. commun. 7, 12071-12071 (2016).
PubMed: 27346279

Assembly members:

Assembly members:
MapZ_SY, polymer, 113 residues, 12299.3762 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETPhos-MapZextra2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MapZ_SY: GHMSRSEVNMGLSSAGVAVQ RSASRVAYNQSAIDDSNNSA WDFADGVLEQILATSRSRGY ITGDQYILERVNIVNGNGYY NLYKPDGTYLFTLNCKTGYF VGNGAGHADDLDY

Data sets:
Data typeCount
13C chemical shifts426
15N chemical shifts113
1H chemical shifts677

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MapZ SY1

Entities:

Entity 1, MapZ SY 113 residues - 12299.3762 Da.

Residues 1 to 3 correspond to the remnant of the Histidine-tag after cleavage.

1   GLYHISMETSERARGSERGLUVALASNMET
2   GLYLEUSERSERALAGLYVALALAVALGLN
3   ARGSERALASERARGVALALATYRASNGLN
4   SERALAILEASPASPSERASNASNSERALA
5   TRPASPPHEALAASPGLYVALLEUGLUGLN
6   ILELEUALATHRSERARGSERARGGLYTYR
7   ILETHRGLYASPGLNTYRILELEUGLUARG
8   VALASNILEVALASNGLYASNGLYTYRTYR
9   ASNLEUTYRLYSPROASPGLYTHRTYRLEU
10   PHETHRLEUASNCYSLYSTHRGLYTYRPHE
11   VALGLYASNGLYALAGLYHISALAASPASP
12   LEUASPTYR

Related Database Links:

UNP Q8DR55
AlphaFold Q8DR55

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks