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PDB ID: 2n8t
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25865
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Spagnol, Gaelle; Kieken, Fabien; Kopanic, Jennifer; Li, Hanjun; Zach, Sydney; Stauch, Kelly; Grosely, Rosslyn; Sorgen, Paul. "Structural Studies of the Nedd4 WW Domains and their Selectivity for the Cx43 Carboxyl-terminus" J. Biol. Chem. 291, 7637-7650 (2016).
PubMed: 26841867
Assembly members:
rNedd4_WW2, polymer, 39 residues, 4436.736 Da.
Cx43CT_peptide, polymer, 14 residues, 1617.726 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-KT
Entity Sequences (FASTA):
rNedd4_WW2: GSSSGLPPGWEEKQDDRGRS
YYVDHNSKTTTWSKPTMQD
Cx43CT_peptide: APLXPMXPPGYKLV
Data type | Count |
13C chemical shifts | 119 |
15N chemical shifts | 35 |
1H chemical shifts | 266 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | rNedd4 WW2 Domain | 1 |
2 | Cx43CT Peptide | 2 |
Entity 1, rNedd4 WW2 Domain 39 residues - 4436.736 Da.
1 | GLY | SER | SER | SER | GLY | LEU | PRO | PRO | GLY | TRP | ||||
2 | GLU | GLU | LYS | GLN | ASP | ASP | ARG | GLY | ARG | SER | ||||
3 | TYR | TYR | VAL | ASP | HIS | ASN | SER | LYS | THR | THR | ||||
4 | THR | TRP | SER | LYS | PRO | THR | MET | GLN | ASP |
Entity 2, Cx43CT Peptide 14 residues - 1617.726 Da.
1 | ALA | PRO | LEU | SEP | PRO | MET | SEP | PRO | PRO | GLY | ||||
2 | TYR | LYS | LEU | VAL |
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