BMRB Entry 25865

Title:
Solution Structure of the rNedd4 WW2 Domain-Cx43CT Peptide Complex by NMR
Deposition date:
2015-10-27
Original release date:
2016-02-22
Authors:
Spagnol, Gaelle; Kieken, Fabien; Sorgen, Paul
Citation:

Citation: Spagnol, Gaelle; Kieken, Fabien; Kopanic, Jennifer; Li, Hanjun; Zach, Sydney; Stauch, Kelly; Grosely, Rosslyn; Sorgen, Paul. "Structural Studies of the Nedd4 WW Domains and their Selectivity for the Cx43 Carboxyl-terminus"  J. Biol. Chem. 291, 7637-7650 (2016).
PubMed: 26841867

Assembly members:

Assembly members:
rNedd4_WW2, polymer, 39 residues, 4436.736 Da.
Cx43CT_peptide, polymer, 14 residues, 1617.726 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rNedd4_WW2: GSSSGLPPGWEEKQDDRGRS YYVDHNSKTTTWSKPTMQD
Cx43CT_peptide: APLXPMXPPGYKLV

Data sets:
Data typeCount
13C chemical shifts119
15N chemical shifts35
1H chemical shifts266

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rNedd4 WW2 Domain1
2Cx43CT Peptide2

Entities:

Entity 1, rNedd4 WW2 Domain 39 residues - 4436.736 Da.

1   GLYSERSERSERGLYLEUPROPROGLYTRP
2   GLUGLULYSGLNASPASPARGGLYARGSER
3   TYRTYRVALASPHISASNSERLYSTHRTHR
4   THRTRPSERLYSPROTHRMETGLNASP

Entity 2, Cx43CT Peptide 14 residues - 1617.726 Da.

1   ALAPROLEUSEPPROMETSEPPROPROGLY
2   TYRLYSLEUVAL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
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