BMRB Entry 25778

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PDB ID: 2n6r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25778
MolProbity Validation Chart

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Title:
Chemical shift assignments and structure calculation of spider toxin U4-hexatoxin-Hi1a
Deposition date:
2015-08-28
Original release date:
2016-08-25
Authors:
Pineda, Sandy; Chin, Yanni; King, Glenn
Citation:

Citation: Pineda, Sandy; Chin, Yanni K-Y; Undheim, Eivind; Senff, Sebastian; Mobli, Mehdi; Dauly, Claire; Escoubas, Pierre; Nicholson, Graham; Kaas, Quentin; Guo, Shaodong; Herzig, Volker; Mattick, John; King, Glenn. "Structural venomics reveals evolution of a complex venom by duplication and diversification of an ancient peptide-encoding gene"  Proc. Natl. Acad. Sci. U. S. A. 117, 11399-11408 (2020).
PubMed: 32398368

Assembly members:

Assembly members:
U4-hexatoxin-Hi1a, polymer, 76 residues, 8821.980 Da.

Natural source:

Natural source:   Common Name: spiders   Taxonomy ID: 153481   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche infensa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLICC vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U4-hexatoxin-Hi1a: GTPSADQVRYNYTELPNGEY CYTPRRRCTSADQCCRPYDT TAAFHGCGRIWPKDKREKVD RCYICNNEKTLCTSVM

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts80
1H chemical shifts502

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1spider toxin U4-hexatoxin-Hi1a1

Entities:

Entity 1, spider toxin U4-hexatoxin-Hi1a 76 residues - 8821.980 Da.

1   GLYTHRPROSERALAASPGLNVALARGTYR
2   ASNTYRTHRGLULEUPROASNGLYGLUTYR
3   CYSTYRTHRPROARGARGARGCYSTHRSER
4   ALAASPGLNCYSCYSARGPROTYRASPTHR
5   THRALAALAPHEHISGLYCYSGLYARGILE
6   TRPPROLYSASPLYSARGGLULYSVALASP
7   ARGCYSTYRILECYSASNASNGLULYSTHR
8   LEUCYSTHRSERVALMET

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