BMRB Entry 25763

Name: Solution structure of an MbtH-like protein from Mycobacterium avium, Seattle Structural Genomics Center for Infectious Disease target MyavA.01649.c
Published: 2015-12-28

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PDB ID: 2n6g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25763
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of an MbtH-like protein from Mycobacterium avium, Seattle Structural Genomics Center for Infectious Disease target MyavA.01649.c

Deposition date:

2015-08-20

Original release date:

2015-12-28

Authors:

Buchko, Garry

Citation:

Citation: Buchko, Garry; Hewitt, Stephen; Van Voorhis, Wesley; Myler, Peter. "Solution structure of a MbtH-like protein from Mycobacterium avium." .

Assembly members:

Assembly members:
entity, polymer, 80 residues, 8994.921 Da.

Natural source:

Natural source: Common Name: high GC Gram+ Taxonomy ID: 1794 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium avium

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPGSMSINPFDDDNGSFFVL VNDEEQHSLWPAFADVPAGW RVVHGEADRAACLEYIEEHW PDIRPKSLRDKLATGRGFDQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	235
15N chemical shifts	62
1H chemical shifts	322

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 80 residues - 8994.921 Da.

The first four residues are non-native and remain after removal of the N-terminal tag containing a poly-histidine site.

1	GLY	PRO	GLY	SER	MET	SER	ILE	ASN	PRO	PHE
2	ASP	ASP	ASP	ASN	GLY	SER	PHE	PHE	VAL	LEU
3	VAL	ASN	ASP	GLU	GLU	GLN	HIS	SER	LEU	TRP
4	PRO	ALA	PHE	ALA	ASP	VAL	PRO	ALA	GLY	TRP
5	ARG	VAL	VAL	HIS	GLY	GLU	ALA	ASP	ARG	ALA
6	ALA	CYS	LEU	GLU	TYR	ILE	GLU	GLU	HIS	TRP
7	PRO	ASP	ILE	ARG	PRO	LYS	SER	LEU	ARG	ASP
8	LYS	LEU	ALA	THR	GLY	ARG	GLY	PHE	ASP	GLN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
deuterium exchange	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 25763

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	PRO	GLY	SER	MET	SER	ILE	ASN	PRO	PHE
2	ASP	ASP	ASP	ASN	GLY	SER	PHE	PHE	VAL	LEU
3	VAL	ASN	ASP	GLU	GLU	GLN	HIS	SER	LEU	TRP
4	PRO	ALA	PHE	ALA	ASP	VAL	PRO	ALA	GLY	TRP
5	ARG	VAL	VAL	HIS	GLY	GLU	ALA	ASP	ARG	ALA
6	ALA	CYS	LEU	GLU	TYR	ILE	GLU	GLU	HIS	TRP
7	PRO	ASP	ILE	ARG	PRO	LYS	SER	LEU	ARG	ASP
8	LYS	LEU	ALA	THR	GLY	ARG	GLY	PHE	ASP	GLN

1	GLY	PRO	GLY	SER	MET	SER	ILE	ASN	PRO	PHE
2	ASP	ASP	ASP	ASN	GLY	SER	PHE	PHE	VAL	LEU
3	VAL	ASN	ASP	GLU	GLU	GLN	HIS	SER	LEU	TRP
4	PRO	ALA	PHE	ALA	ASP	VAL	PRO	ALA	GLY	TRP
5	ARG	VAL	VAL	HIS	GLY	GLU	ALA	ASP	ARG	ALA
6	ALA	CYS	LEU	GLU	TYR	ILE	GLU	GLU	HIS	TRP
7	PRO	ASP	ILE	ARG	PRO	LYS	SER	LEU	ARG	ASP
8	LYS	LEU	ALA	THR	GLY	ARG	GLY	PHE	ASP	GLN

1	GLY	PRO	GLY	SER	MET	SER	ILE	ASN	PRO	PHE
2	ASP	ASP	ASP	ASN	GLY	SER	PHE	PHE	VAL	LEU
3	VAL	ASN	ASP	GLU	GLU	GLN	HIS	SER	LEU	TRP
4	PRO	ALA	PHE	ALA	ASP	VAL	PRO	ALA	GLY	TRP
5	ARG	VAL	VAL	HIS	GLY	GLU	ALA	ASP	ARG	ALA
6	ALA	CYS	LEU	GLU	TYR	ILE	GLU	GLU	HIS	TRP
7	PRO	ASP	ILE	ARG	PRO	LYS	SER	LEU	ARG	ASP
8	LYS	LEU	ALA	THR	GLY	ARG	GLY	PHE	ASP	GLN