BMRB Entry 25677

Name: Solution NMR structure of CBX8 in complex with AF9 (CBX8-AF9)
Published: 2016-07-18

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PDB ID: 2n4q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25677
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of CBX8 in complex with AF9 (CBX8-AF9)

Deposition date:

2015-06-30

Original release date:

2016-07-18

Authors:

Kuntimaddi, Aravinda; Leach, Benjamin; Bushweller, John

Citation:

Citation: Kuntimaddi, Aravinda; Leach, Benjamin; Bushweller, John. "Solution NMR Structure of CBX8 in complex with AF9 (CBX8-AF9)" .

Assembly members:

Assembly members:
Chromobox_protein_homolog_8, polymer, 24 residues, 2577.936 Da.
Protein_AF-9, polymer, 70 residues, 8278.482 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PETDUET-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chromobox_protein_homolog_8: TQGGRPSLIARIPVARILGD PEEE
Protein_AF-9: MDKAYLDELVELHRRLMTLR ERHILQQIVNLIEETGHFHI TNTTFDFDLCSLDKTTVRKL QSYLETSGTS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	406
15N chemical shifts	88
1H chemical shifts	542

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 24 residues - 2577.936 Da.

RESIDUES 327-349

1

THR

GLN

GLY

ARG

PRO

SER

LEU

ILE

ALA

2

ARG

ILE

PRO

VAL

ALA

ARG

ILE

LEU

GLY

ASP

3

PRO

GLU

Entity 2, entity_2 70 residues - 8278.482 Da.

RESIDUES 500-568

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

UNP	Q9HC52 P42568
AlphaFold	Q9NR07 Q8IVB0

BMRB Entry 25677

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER

1	MET	ASP	LYS	ALA	TYR	LEU	ASP	GLU	LEU	VAL
2	GLU	LEU	HIS	ARG	ARG	LEU	MET	THR	LEU	ARG
3	GLU	ARG	HIS	ILE	LEU	GLN	GLN	ILE	VAL	ASN
4	LEU	ILE	GLU	GLU	THR	GLY	HIS	PHE	HIS	ILE
5	THR	ASN	THR	THR	PHE	ASP	PHE	ASP	LEU	CYS
6	SER	LEU	ASP	LYS	THR	THR	VAL	ARG	LYS	LEU
7	GLN	SER	TYR	LEU	GLU	THR	SER	GLY	THR	SER