BMRB Entry 25542

Name: Solution structure of MyUb (1080-1131) of human Myosin VI
Published: 2016-03-07

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PDB ID: 2n10
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25542
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of MyUb (1080-1131) of human Myosin VI

Deposition date:

2015-03-20

Original release date:

2016-03-07

Authors:

He, Fahu; Walters, Kylie; Ehlinger, Aaron

Citation:

Citation: He, Fahu; Wollscheid, Hans-Peter; Nowicka, Urszula; Biancospino, Matteo; Valentini, Eleonora; Ehlinger, Aaron; Acconcia, Filippo; Magistrati, Elisa; Polo, Simona; Walters, Kylie. "Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains" Cell Rep. 14, 2683-2694 (2016).
PubMed: 26971995

Assembly members:

Assembly members:
entity, polymer, 60 residues, 6345.419 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6p

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPLGSPNSGTKKYDLSKWKY AELRDTINTSCDIELLAACR EEFHRRLKVYHAWKSKNKKR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	273
15N chemical shifts	64
1H chemical shifts	429

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 60 residues - 6345.419 Da.

The first 8 residues (GPLGSPNS) are a tag

1	GLY	PRO	LEU	GLY	SER	PRO	ASN	SER	GLY	THR
2	LYS	LYS	TYR	ASP	LEU	SER	LYS	TRP	LYS	TYR
3	ALA	GLU	LEU	ARG	ASP	THR	ILE	ASN	THR	SER
4	CYS	ASP	ILE	GLU	LEU	LEU	ALA	ALA	CYS	ARG
5	GLU	GLU	PHE	HIS	ARG	ARG	LEU	LYS	VAL	TYR
6	HIS	ALA	TRP	LYS	SER	LYS	ASN	LYS	LYS	ARG

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Uniprot	Q9UM54
AlphaFold	Q9UEG2

BMRB Entry 25542

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	PRO	LEU	GLY	SER	PRO	ASN	SER	GLY	THR
2	LYS	LYS	TYR	ASP	LEU	SER	LYS	TRP	LYS	TYR
3	ALA	GLU	LEU	ARG	ASP	THR	ILE	ASN	THR	SER
4	CYS	ASP	ILE	GLU	LEU	LEU	ALA	ALA	CYS	ARG
5	GLU	GLU	PHE	HIS	ARG	ARG	LEU	LYS	VAL	TYR
6	HIS	ALA	TRP	LYS	SER	LYS	ASN	LYS	LYS	ARG

1	GLY	PRO	LEU	GLY	SER	PRO	ASN	SER	GLY	THR
2	LYS	LYS	TYR	ASP	LEU	SER	LYS	TRP	LYS	TYR
3	ALA	GLU	LEU	ARG	ASP	THR	ILE	ASN	THR	SER
4	CYS	ASP	ILE	GLU	LEU	LEU	ALA	ALA	CYS	ARG
5	GLU	GLU	PHE	HIS	ARG	ARG	LEU	LYS	VAL	TYR
6	HIS	ALA	TRP	LYS	SER	LYS	ASN	LYS	LYS	ARG

1	GLY	PRO	LEU	GLY	SER	PRO	ASN	SER	GLY	THR
2	LYS	LYS	TYR	ASP	LEU	SER	LYS	TRP	LYS	TYR
3	ALA	GLU	LEU	ARG	ASP	THR	ILE	ASN	THR	SER
4	CYS	ASP	ILE	GLU	LEU	LEU	ALA	ALA	CYS	ARG
5	GLU	GLU	PHE	HIS	ARG	ARG	LEU	LYS	VAL	TYR
6	HIS	ALA	TRP	LYS	SER	LYS	ASN	LYS	LYS	ARG