BMRB Entry 25461

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PDB ID: 2myx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25461
MolProbity Validation Chart

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Title:
Structure of the CUE domain of yeast Cue1
Deposition date:
2015-02-02
Original release date:
2016-03-21
Authors:
Kniss, Andreas; Rogov, Vladimir; Loehr, Frank; Guentert, Peter; Doetsch, Volker
Citation:

Citation: von Delbrueck, Maximilian; Kniss, Andreas; Rogov, Vladimir; Pluska, Lukas; Bagola, Katrin; Loehr, Frank; Guentert, Peter; Sommer, Thomas; Doetsch, Volker. "Dynamic binding of a ubiquitin binding domain to polyubiquitin stimulates chain elongation by an E2 enzyme"  .

Assembly members:

Assembly members:
Coupling_of_ubiquitin_conjugation_to_ER_degradation_protein_1_(Cue1), polymer, 75 residues, 8266.121 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET39(b)+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Coupling_of_ubiquitin_conjugation_to_ER_degradation_protein_1_(Cue1): GAMGPQGSTQNALRRTGRVN GGHPVTTQMVETVQNLAPNL HPEQIRYSLENTGSVEETVE RYLRGDEFSFPPGFE

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts81
1H chemical shifts498

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 75 residues - 8266.121 Da.

Residues 41-44 represent non-native residues (cloning artifacts). Residues 45-115 represent native amino acids of yeast Cue1.

1   GLYALAMETGLYPROGLNGLYSERTHRGLN
2   ASNALALEUARGARGTHRGLYARGVALASN
3   GLYGLYHISPROVALTHRTHRGLNMETVAL
4   GLUTHRVALGLNASNLEUALAPROASNLEU
5   HISPROGLUGLNILEARGTYRSERLEUGLU
6   ASNTHRGLYSERVALGLUGLUTHRVALGLU
7   ARGTYRLEUARGGLYASPGLUPHESERPHE
8   PROPROGLYPHEGLU

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks