BMRB Entry 25407
Click here to enlarge.
PDB ID: 2mxf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25407
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ding, Pengfei; McFarland, Kirsty; Jin, Shujuan; Tong, Grace; Duan, Bo; Yang, Ally; Hughes, Timothy; Liu, Jun; Dove, Simon; Navarre, William; Xia, Bin. "A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT" Plos Pathog. 11, e1004967-e1004967 (2015).
PubMed: 26068099
Assembly members:
MvaT, polymer, 55 residues, 5352.173 Da.
DNA_(5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3'), polymer, 12 residues, 3662.434 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b
Entity Sequences (FASTA):
MvaT: MKRARKVKQYKNPHTGEVIE
TKGGNHKTLKEWKAKWGPEA
VESWATLLGHHHHHH
DNA_(5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3'): CGCATATATGCG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 163 |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 536 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MvaT | 1 |
| 2 | DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')_1 | 2 |
| 3 | DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')_2 | 2 |
Entities:
Entity 1, MvaT 55 residues - 5352.173 Da.
| 1 | MET | LYS | ARG | ALA | ARG | LYS | VAL | LYS | GLN | TYR | ||||
| 2 | LYS | ASN | PRO | HIS | THR | GLY | GLU | VAL | ILE | GLU | ||||
| 3 | THR | LYS | GLY | GLY | ASN | HIS | LYS | THR | LEU | LYS | ||||
| 4 | GLU | TRP | LYS | ALA | LYS | TRP | GLY | PRO | GLU | ALA | ||||
| 5 | VAL | GLU | SER | TRP | ALA | THR | LEU | LEU | GLY | HIS | ||||
| 6 | HIS | HIS | HIS | HIS | HIS |
Entity 2, DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')_1 12 residues - 3662.434 Da.
| 1 | DC | DG | DC | DA | DT | DA | DT | DA | DT | DG | ||||
| 2 | DC | DG |
Related Database Links:
| BMRB | 25405 |
| PDB | 2MXE 2MXF |
| DBJ | BAK88160 BAP21250 BAP52811 BAQ41953 BAR69621 |
| EMBL | CAW29447 CCQ87551 CDH72901 CDH79198 CDI92830 |
| GB | AAG07703 AAP33788 ABJ13585 ABR84836 AEO76886 |
| REF | NP_253005 WP_003093888 WP_003125172 WP_012077126 WP_015478773 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks