BMRB Entry 25149

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PDB ID: 2mt5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25149
MolProbity Validation Chart

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Title:
Isolated Ring domain
Deposition date:
2014-08-13
Original release date:
2014-10-27
Authors:
Brown, Nicholas; Watson, Edmond; Weissman, Florian; Royappa, Grace; Schulman, Brenda; Jarvis, Marc; Vanderlinden, Ryan; Frye, Jeremiah; Qiao, Renping; Petzold, Georg; Peters, Jan-Michael; Stark, Holger
Citation:

Citation: Brown, Nicholas; Watson, Edmond; Weissman, Florian; Royappa, Grace; Jarvis, Marc; Vanderlinden, Ryan; Frye, Jeremiah; Qiao, Renping; Petzold, Georg; Peters, Jan-Michael; Stark, Holger; Schulman, Brenda. "Mechanism of Polyubiquitination by Human Anaphase-Promoting Complex: RING Repurposing for Ubiquitin Chain Assembly"  Mol. Cell ., .-. (2014).
PubMed: 25306923

Assembly members:

Assembly members:
entity_1, polymer, 70 residues, 8191.060 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: GST-Fusion with C-terminal Cys-His6tag

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSVANDENCGICRMAFNGCC PDCKVPGDDCPLVWGQCSHC FHMHCILKWLHAQQVQQHCP MCRQEWKFKE

Data sets:
Data typeCount
1H chemical shifts469
13C chemical shifts292
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2Zinc Ion 12
3Zinc Ion 22
4Zinc Ion 32

Entities:

Entity 1, entity_1 70 residues - 8191.060 Da.

1   GLYSERVALALAASNASPGLUASNCYSGLY
2   ILECYSARGMETALAPHEASNGLYCYSCYS
3   PROASPCYSLYSVALPROGLYASPASPCYS
4   PROLEUVALTRPGLYGLNCYSSERHISCYS
5   PHEHISMETHISCYSILELEULYSTRPLEU
6   HISALAGLNGLNVALGLNGLNHISCYSPRO
7   METCYSARGGLNGLUTRPLYSPHELYSGLU

Entity 2, Zinc Ion 1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2MT5 4R2Y 4UI9
DBJ BAB22663 BAB22890 BAB22937 BAC32348 BAE22944
EMBL CAH92008
GB AAF65816 AAH23039 AAH66308 AAH95454 AAI02427
REF NP_001002245 NP_001002246 NP_001002247 NP_001002248 NP_001002249
SP Q3ZCF6 Q5R8A2 Q9CPX9 Q9NYG5
TPG DAA14168 DAA18262
AlphaFold Q3ZCF6 Q5R8A2 Q9CPX9 Q9NYG5

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