BMRB Entry 19403

Assembly:

Entities:

Entity 1, Clip-segment of the von Willebrand domain 1 of Crossveinless 2 67 residues - 7192.319 Da.

residues L1 to G66 represent the first von Willebrand type C domain of Danio rerio Crossveinless 2. Numbering refers to mature part.

Samples:

sample_1: CV2 VWC1, [U-99% 13C; U-99% 15N], 2 mM; sodium phosphate 20 mM; sodium chloride 20 mM; D2O, [U-2H], 5%; sodium azide 0.2%; H2O, [U-2H], 95%

sample_2: CV2 VWC1, [U-99% 15N], 1.5 mM; sodium phosphate 20 mM; sodium chloride 20 mM; D2O, [U-2H], 5%; sodium azide 0.2%; H2O, [U-2H], 95%

sample_3: CV2 VWC1 2 mM; sodium phosphate 20 mM; sodium chloride 20 mM; D2O, [U-2H], 5%; sodium azide 0.2%; H2O, [U-2H], 95%

sample_conditions_1: ionic strength: 0.04 M; pH: 6.6; pressure: 1 atm; temperature: 300 K

Experiments:

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

AURELIA v3.85, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - chemical shift assignment, data analysis

X-PLOR NIH v2.29, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

• Bruker Avance 600 MHz
• Bruker Avance 900 MHz