BMRB Entry 19299

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PDB ID: 2m9u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19299
MolProbity Validation Chart

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Title:
Analysis of target DNA binding sites through NMR structural analysis of the MLV IN CTD and homology modeling of the CCD domains.
Deposition date:
2013-06-12
Original release date:
2013-12-16
Authors:
Aiyer, Sriram; Schneider, William; Chander, Ashwin; Roth, Monica; Montelione, Gaetano; Genomics Consortium, Northeast Structural; Rossi, Paolo
Citation:

Citation: Aiyer, Sriram; Schneider, William; Chander, Ashwin; Montelione, Gaetano; Roth, Monica; Rossi, Paolo. "Analysis of target DNA binding sites through NMR structural analysis of the MLV IN CTD and homology modeling of the CCD domains."  .

Assembly members:

Assembly members:
OR41A-15.1, polymer, 91 residues, 10239.74 Da.

Natural source:

Natural source:   Common Name: Murine leukemia virus   Taxonomy ID: 11786   Superkingdom: Viruses   Kingdom: not available   Genus/species: Gammaretrovirus Murine leukemia virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OR41A-15.1: MGHHHHHHSHMVGDTVWVRR HQTKNLEPRWKGPYTVLLTT PTALKVDGIAAWIHAAHVKA ADPGGGPSSRLTWRVQRSQN PLKIRLTREAP

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts74
1H chemical shifts492

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MLV CTD IN1

Entities:

Entity 1, MLV CTD IN 91 residues - 10239.74 Da.

The sequence used to solve the structure is a construct (329-408 amino acids) of the the full length Moloney murine leukemia virus integrase protein (IN)(408 amino acids). The construct has a non-cleavable tag at the N terminus (M G H H H H H H S H M) that is used for purification.

1   METGLYHISHISHISHISHISHISSERHIS
2   METVALGLYASPTHRVALTRPVALARGARG
3   HISGLNTHRLYSASNLEUGLUPROARGTRP
4   LYSGLYPROTYRTHRVALLEULEUTHRTHR
5   PROTHRALALEULYSVALASPGLYILEALA
6   ALATRPILEHISALAALAHISVALLYSALA
7   ALAASPPROGLYGLYGLYPROSERSERARG
8   LEUTHRTRPARGVALGLNARGSERGLNASN
9   PROLEULYSILEARGLEUTHRARGGLUALA
10   PRO

Related Database Links:

PDB 2M9U

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