BMRB Entry 19049

Title:
Solution structure of chicken Engrailed 2 homeodomain
Deposition date:
2013-02-21
Original release date:
2013-08-14
Authors:
Carlier, Ludovic; Balayssac, Stephane; Cantrelle, Francois-Xavier; Khemtemourian, Lucie; Chassaing, Gerard; Joliot, Alain; Lequin, Olivier
Citation:

Citation: Carlier, Ludovic; Balayssac, Stephane; Cantrelle, Francois-Xavier; Khemtemourian, Ludovic; Chassaing, Gerard; Joliot, Alain; Lequin, Olivier. "Investigation of homeodomain membrane translocation properties: insights from the structure determination of engrailed-2 homeodomain in aqueous and membrane-mimetic environments"  Biophys. J. 105, 667-678 (2013).
PubMed: 23931315

Assembly members:

Assembly members:
HOMEOBOX_PROTEIN_ENGRAILED-2, polymer, 67 residues, 7857.0148 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HOMEOBOX_PROTEIN_ENGRAILED-2: GPMASDKRPRTAFTAEQLQR LKAEFQTNRYLTEQRRQSLA QELGLNESQIKIWFQNKRAK IKKATQA

Data sets:
Data typeCount
13C chemical shifts332
15N chemical shifts85
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HOMEOBOX PROTEIN ENGRAILED-21

Entities:

Entity 1, HOMEOBOX PROTEIN ENGRAILED-2 67 residues - 7857.0148 Da.

1   GLYPROMETALASERASPLYSARGPROARG
2   THRALAPHETHRALAGLUGLNLEUGLNARG
3   LEULYSALAGLUPHEGLNTHRASNARGTYR
4   LEUTHRGLUGLNARGARGGLNSERLEUALA
5   GLNGLULEUGLYLEUASNGLUSERGLNILE
6   LYSILETRPPHEGLNASNLYSARGALALYS
7   ILELYSLYSALATHRGLNALA

Related Database Links:

UNP HME2_CHICK
BMRB 17325
PDB 3ZOB
DBJ BAE34186
EMBL CAA44724 CAA48254 CAA48491 CAA68362
GB AAA53438 AAA53504 AAA53527 AAF31303 AAF68670
PRF 1703276B 1703276F
REF NP_001095261 NP_001102684 NP_001254648 NP_001418 NP_034264
SP P09015 P09066 P19622 P52730 Q05917
TPG DAA30339
AlphaFold Q05917 P09015 P09066 P19622 P52730 Q05917

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks