BMRB Entry 18816

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PDB ID: 2m0p
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18816
MolProbity Validation Chart

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Title:
Solution structure of the tenth complement type repeat of human megalin
Deposition date:
2012-11-01
Original release date:
2013-01-07
Authors:
Dagil, Robert; Kragelund, Birthe
Citation:

Citation: Dagil, Robert; Nykjaer, Charlotte; Bonvin, Anders; Kragelund, Alexandre M J J. "Gentamicin binds to the megalin receptor as a competitive inhibitor using the common ligand binding motif of complement type repeats: insight from the nmr structure of the 10th complement type repeat domain alone and in complex with gentamicin."  J. Biol. Chem. 288, 4424-4435 (2013).
PubMed: 23275343

Assembly members:

Assembly members:
CR10, polymer, 52 residues, 5045.292 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICaC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CR10: THAPASCLDTQYTCDNHQCI SKNWVCDTDNDCGDGSDEKN CNSTETHHHHHH

Data sets:
Data typeCount
13C chemical shifts164
15N chemical shifts44
1H chemical shifts243

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CR101
2Calcium ion2

Entities:

Entity 1, CR10 52 residues - 5045.292 Da.

1   THRHISALAPROALASERCYSLEUASPTHR
2   GLNTYRTHRCYSASPASNHISGLNCYSILE
3   SERLYSASNTRPVALCYSASPTHRASPASN
4   ASPCYSGLYASPGLYSERASPGLULYSASN
5   CYSASNSERTHRGLUTHRHISHISHISHIS
6   HISHIS

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Related Database Links:

PDB 2M0P

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