BMRB Entry 18789

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PDB ID: 2m01
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18789
MolProbity Validation Chart

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Title:
Solution structure of Kunitz-type neurotoxin LmKKT-1a from scorpion venom
Deposition date:
2012-10-15
Original release date:
2013-11-11
Authors:
Luo, Fan; Jiang, Ling; Liu, Maili; Chen, Zongyun; Wu, Yingliang
Citation:

Citation: Chen, Zongyun; Luo, Fan; Feng, Jing; Yang, Weishan; Zeng, Danyun; Zhao, Ruiming; Cao, Zhijian; Liu, Maili; Li, Wenxin; Jiang, Ling; Wu, Yingliang. "Genomic and structural characterization of Kunitz-type peptide LmKTT-1a highlights diversity and evolution of scorpion potassium channel toxins"  Plos One 8, e60201-e60201 (2013).
PubMed: 23573241

Assembly members:

Assembly members:
LmKKT-1a, polymer, 59 residues, 6510.300 Da.

Natural source:

Natural source:   Common Name: Chinese striped bark scorpion; Vietnamese brown scorpion   Taxonomy ID: 172552   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lychas mucronatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LmKKT-1a: KKKCQLPSDVGKGKASFTRY YYNEESGKCETFIYGGVGGN SNNFLTKEDCCRECAQGSC

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts58
1H chemical shifts334

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1LmKKT-1a1

Entities:

Entity 1, LmKKT-1a 59 residues - 6510.300 Da.

The recombinant protein contain 79 residues ,but residues 1-20 represent a tag, so we renumber the last 59 residues from 1.

1   LYSLYSLYSCYSGLNLEUPROSERASPVAL
2   GLYLYSGLYLYSALASERPHETHRARGTYR
3   TYRTYRASNGLUGLUSERGLYLYSCYSGLU
4   THRPHEILETYRGLYGLYVALGLYGLYASN
5   SERASNASNPHELEUTHRLYSGLUASPCYS
6   CYSARGGLUCYSALAGLNGLYSERCYS

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