BMRB Entry 18677

Click here to enlarge.

PDB ID: 2lxh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18677
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C and 15N Assignments of the RING domain in ubiquitin ligase gp78
Deposition date:
2012-08-27
Original release date:
2013-08-26
Authors:
Das, Ranabir; Linag, Yuhe; Mariano, Jennifer; Li, Jess; Huang, Tao; King, Aaren; Weissman, Allan; Ji, Xinhua; Byrd, R. Andrew
Citation:

Citation: Das, Ranabir; Liang, Yu-He; Mariano, Jennifer; Li, Jess; Huang, Tao; King, Aaren; Tarasov, Sergey; Weissman, Allan; Ji, Xinhua; Byrd, R. Andrew. "Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine."  EMBO J. 32, 2504-2516 (2013).
PubMed: 23942235

Assembly members:

Assembly members:
gp78RING, polymer, 58 residues, 6473.385 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gp78RING: AVATPEELAVNNDDCAICWD SMQAARKLPCGHLFHNSCLR SWLEQDTSCPTCRMSLNI

Data sets:
Data typeCount
13C chemical shifts232
15N chemical shifts70
1H chemical shifts352

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING domain in ubiquitin ligase gp781
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, RING domain in ubiquitin ligase gp78 58 residues - 6473.385 Da.

1   ALAVALALATHRPROGLUGLULEUALAVAL
2   ASNASNASPASPCYSALAILECYSTRPASP
3   SERMETGLNALAALAARGLYSLEUPROCYS
4   GLYHISLEUPHEHISASNSERCYSLEUARG
5   SERTRPLEUGLUGLNASPTHRSERCYSPRO
6   THRCYSARGMETSERLEUASNILE

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2LXH 2LXP 4LAD
DBJ BAE01277 BAE34049 BAE41974 BAE87377 BAK63135
GB AAD56721 AAD56722 AAH17043 AAH34538 AAH40338
REF NP_001039439 NP_001135 NP_001267243 NP_035917 XP_001091030
SP Q9R049 Q9UKV5
TPG DAA20037
AlphaFold Q9R049 Q9UKV5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks