BMRB Entry 18642

Click here to enlarge.

PDB ID: 2lwr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18642
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Protein structure
Deposition date:
2012-08-06
Original release date:
2013-06-10
Authors:
Mercier, Pascal; Spratt, Donald; Manczyk, Noah; Shaw, Gary
Citation:

Citation: Spratt, Donald; Julio Martinez-Torres, R.; Noh, Yeong; Mercier, Pascal; Manczyk, Noah; Barber, Kathryn; Aguirre, Jacob; Burchell, Lynn; Purkiss, Andrew; Walden, Helen; Shaw, Gary. "A molecular explanation for the recessive nature of parkin-linked Parkinson's disease."  Nat. Commun. 4, 1983-1983 (2013).
PubMed: 23770917

Assembly members:

Assembly members:
Protein, polymer, 68 residues, 7669.769 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein: GSAEARWDEASNVTIKVSTK PCPKCRTPTERDGGCMHMVC TRAGCGFEWCWVCQTEWTRD CMGAHWFG

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts63
1H chemical shifts388

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein1
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, Protein 68 residues - 7669.769 Da.

N-terminal GS sequence results from the cleavage site

1   GLYSERALAGLUALAARGTRPASPGLUALA
2   SERASNVALTHRILELYSVALSERTHRLYS
3   PROCYSPROLYSCYSARGTHRPROTHRGLU
4   ARGASPGLYGLYCYSMETHISMETVALCYS
5   THRARGALAGLYCYSGLYPHEGLUTRPCYS
6   TRPVALCYSGLNTHRGLUTRPTHRARGASP
7   CYSMETGLYALAHISTRPPHEGLY

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 18990
PDB 2LWR 2M48
GB AAL13983 AAM18800 AAM43930 AAN12154 AAN12155
REF NP_730600 NP_730601 XP_001956965 XP_001973584 XP_001984548

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks