BMRB Entry 18358

Title:
The solution structure of the dimeric Acanthaporin
Deposition date:
2012-03-28
Original release date:
2012-05-01
Authors:
Michalek, Matthias; Soennichsen, Frank; Wechselberger, Rainer; Dingley, Andrew; Wienk, Hans; Simanski, Maren; Herbst, Rosa; Lorenzen, Inken; Marciano-Cabral, Francine; Gelhaus, Christoph; Groetzinger, Joachim; Leippe, Matthias
Citation:

Citation: Michalek, Matthias; Soennichsen, Frank; Wechselberger, Rainer; Dingley, Andrew; Wienk, Hans; Simanski, Maren; Herbst, Rosa; Lorenzen, Inken; Marciano-Cabral, Francine; Gelhaus, Christoph; Groetzinger, Joachim; Leippe, Matthias. "The solution structure of the dimeric Acanthaporin"  .

Assembly members:

Assembly members:
acanthaporin_dimer, polymer, 61 residues, 6001.235 Da.

Natural source:

Natural source:   Common Name: Acanthamoeba culbertsoni   Taxonomy ID: 43142   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Acanthamoeba culbertsoni

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
acanthaporin_dimer: AMGKCSVLKKVACAAAIAGA VAACGGIDLPCVLAALKAAE GCASCFCEDHCHGVCKDLHL C

Data sets:
Data typeCount
15N chemical shifts43
1H chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acanthaporin_dimer, chain 11
2acanthaporin_dimer, chain 21

Entities:

Entity 1, acanthaporin_dimer, chain 1 61 residues - 6001.235 Da.

1   ALAMETGLYLYSCYSSERVALLEULYSLYS
2   VALALACYSALAALAALAILEALAGLYALA
3   VALALAALACYSGLYGLYILEASPLEUPRO
4   CYSVALLEUALAALALEULYSALAALAGLU
5   GLYCYSALASERCYSPHECYSGLUASPHIS
6   CYSHISGLYVALCYSLYSASPLEUHISLEU
7   CYS

Samples:

sample_1: acanthaporin_dimer, [U-100% 15N], 0.7 mM; TRIS, [U-2H], 25 mM; sodium azide 0.001%; H2O 93%; D2O 7%

sample_2: acanthaporin_dimer, [U-100% 15N], 0.7 mM; TRIS, [U-2H], 25 mM; sodium azide 0.001%; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.025 M; pH: 8.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz

Related Database Links:

BMRB 16727 16819 18357
PDB
GB AEC33273

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks