BMRB Entry 18328

Assembly:

Entities:

Entity 1, CHCHD7 85 residues - 8598.808 Da.

The first 13 residues are removed from the coordinate file as they are disordered. Two disulfide bonds are present between Cys16 and Cys47, and between Cys26 and Cys37

Samples:

sample_1: CHCHD7, [U-100% 15N], 0.5 – 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_2: CHCHD7, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - data analysis

PECAN, Eghbalnia, Wang, Bahrami, Assadi, and Markley - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

ATNOS, Herrmann, Guntert and Wuthrich - peak picking

CANDID, Herrmann, Guntert and Wuthrich - NOEs assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

XEASY, Bartels et al. - data analysis

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

ProcheckNMR, Laskowski and MacArthur - Structure validation

PSVS, Bhattacharya and Montelione - Structure validation

WhatIF, Vriend - Structure validation

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

• Bruker Avance 900 MHz
• Bruker Avance 500 MHz
• Bruker Avance 700 MHz