BMRB Entry 18084

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PDB ID: 2llq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18084
MolProbity Validation Chart

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Title:
SOLUTION NMR-DERIVED STRUCTURE OF CALMODULIN C-LOBE BOUND WITH ER ALPHA PEPTIDE
Deposition date:
2011-11-15
Original release date:
2012-01-30
Authors:
Zhang, Yonghong
Citation:

Citation: Zhang, Yonghong; Li, Zhigang; Sacks, David; Ames, James. "Structural basis for Ca2+-induced activation and dimerization of estrogen receptor by calmodulin."  J. Biol. Chem. 287, 9336-9344 (2012).
PubMed: 22275375

Assembly members:

Assembly members:
Calmodulin C-Lobe, polymer, 67 residues, 7737.527 Da.
ER alpha peptide, polymer, 19 residues, 2202.663 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calmodulin C-Lobe: EEEIREAFRVFDKDGNGYIS AAELRHVMTNLGEKLTDEEV DEMIREADIDGDGQVNYEEF VQMMTAK
ER alpha peptide: RAANLWPSPLMIKRSKKNS

Data sets:
Data typeCount
13C chemical shifts129
15N chemical shifts67
1H chemical shifts254

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin C-Lobe1
2ER alpha peptide2
3CA2_13
4CA2_23

Entities:

Entity 1, Calmodulin C-Lobe 67 residues - 7737.527 Da.

1   GLUGLUGLUILEARGGLUALAPHEARGVAL
2   PHEASPLYSASPGLYASNGLYTYRILESER
3   ALAALAGLULEUARGHISVALMETTHRASN
4   LEUGLYGLULYSLEUTHRASPGLUGLUVAL
5   ASPGLUMETILEARGGLUALAASPILEASP
6   GLYASPGLYGLNVALASNTYRGLUGLUPHE
7   VALGLNMETMETTHRALALYS

Entity 2, ER alpha peptide 19 residues - 2202.663 Da.

1   ARGALAALAASNLEUTRPPROSERPROLEU
2   METILELYSARGSERLYSLYSASNSER

Entity 3, CA2_1 - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 11440 16994 17881 17981 17982 17983 18323 19376 25344
PDB 1CMF 1CMG 1F71 1FW4 1J7P 1YRT 1YRU 1ZOT 2COL 2K3S 2KUH 2KZ2 2LLQ 2LQP 2RRT 4BYA 4RJD
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566
GB AAB25484 AAH10730 AAO17827 AAQ14324 AAX61134
REF XP_001687795 XP_001869425 XP_007442524 XP_009577260 XP_010190430
AlphaFold Q9UIS7

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