BMRB Entry 17973

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PDB ID: 2lt5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17973
MolProbity Validation Chart

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Title:
Onconase zymogen FLG variant
Deposition date:
2011-10-04
Original release date:
2012-03-09
Authors:
Vilanova, Maria; Callis, Mariona; Laurents, Douglas; Ribo, Marc; Bruix, Marta; Serrano, Soraya
Citation:

Citation: Serrano, Soraya; Callis, Mariona; Vilanova, Maria; Benito, Antoni; Laurents, Douglas; Ribo, Marc; Bruix, Marta. "(1)H, (13)C and (15)N resonance assignments of the Onconase FL-G zymogen."  Biomol. NMR Assignments 7, 13-15 (2013).
PubMed: 22392335

Assembly members:

Assembly members:
onconase_FLG_variant, polymer, 120 residues, 13225.1 Da.

Natural source:

Natural source:   Common Name: Northern leopard frog   Taxonomy ID: 8404   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana pipiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
onconase_FLG_variant: RPCKYKLKKSTNKFCVTCEN QAPVHFVGVGSCGSGGSGIF LETSLSAGSDWLTFQKKHIT NTRDVDCXNIMSTNLFHCKD KNTFIYSRPEPVKAICKGII ASKNVLTTSEFYLSDCNVTS

Data sets:
Data typeCount
13C chemical shifts495
15N chemical shifts125
1H chemical shifts816

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1onconase FLG variant1

Entities:

Entity 1, onconase FLG variant 120 residues - 13225.1 Da.

It's a circular permutated variant of wt onconase. Insertion of 16 amino acids linking the wt N- and C-termini. N-and C-termini of this variant correspond to positions 73 and 72 in the wt onconase.

1   ARGPROCYSLYSTYRLYSLEULYSLYSSER
2   THRASNLYSPHECYSVALTHRCYSGLUASN
3   GLNALAPROVALHISPHEVALGLYVALGLY
4   SERCYSGLYSERGLYGLYSERGLYILEPHE
5   LEUGLUTHRSERLEUSERALAGLYSERASP
6   TRPLEUTHRPHEGLNLYSLYSHISILETHR
7   ASNTHRARGASPVALASPCYSDASASNILE
8   METSERTHRASNLEUPHEHISCYSLYSASP
9   LYSASNTHRPHEILETYRSERARGPROGLU
10   PROVALLYSALAILECYSLYSGLYILEILE
11   ALASERLYSASNVALLEUTHRTHRSERGLU
12   PHETYRLEUSERASPCYSASNVALTHRSER

Related Database Links:

BMRB 4173 5835 16040
PDB 1ONC 1PU3 1YV4 1YV6 1YV7 2GMK 2I5S 2KB6 3FD7 3HG6 3PHM 2LT5
GB AAL54383
SP P22069
AlphaFold P22069

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