BMRB Entry 17785
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17785
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Citation: Buhrman, Greg; O'Connor, Casey; Zerbe, Brandon; Kearney, Bradley; Napoleon, Raeanne; Kovrigina, Elizaveta; Vajda, Sandor; Kozakov, Dima; Kovrigin, Evgenii; Mattos, Carla. "Analysis of binding site hot spots on the surface of Ras GTPase" J. Mol. Biol. 413, .-773 (789).
PubMed: 21945529
Assembly members:
KRas171, polymer, 171 residues, 19464.02 Da.
GNP, non-polymer, 522.196 Da.
MG, non-polymer, 24.305 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 123 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | G-domain | 1 |
| 2 | GNP | 2 |
| 3 | Mg | 3 |
Entities:
Entity 1, G-domain 171 residues - 19464.02 Da.
| 1 | MET | THR | GLU | TYR | LYS | LEU | VAL | VAL | VAL | GLY | ||||
| 2 | ALA | GLY | GLY | VAL | GLY | LYS | SER | ALA | LEU | THR | ||||
| 3 | ILE | GLN | LEU | ILE | GLN | ASN | HIS | PHE | VAL | ASP | ||||
| 4 | GLU | TYR | ASP | PRO | THR | ILE | GLU | ASP | SER | TYR | ||||
| 5 | ARG | LYS | GLN | VAL | VAL | ILE | ASP | GLY | GLU | THR | ||||
| 6 | CYS | LEU | LEU | ASP | ILE | LEU | ASP | THR | ALA | GLY | ||||
| 7 | GLN | GLU | GLU | TYR | SER | ALA | MET | ARG | ASP | GLN | ||||
| 8 | TYR | MET | ARG | THR | GLY | GLU | GLY | PHE | LEU | CYS | ||||
| 9 | VAL | PHE | ALA | ILE | ASN | ASN | THR | LYS | SER | PHE | ||||
| 10 | GLU | ASP | ILE | HIS | HIS | TYR | ARG | GLU | GLN | ILE | ||||
| 11 | LYS | ARG | VAL | LYS | ASP | SER | GLU | ASP | VAL | PRO | ||||
| 12 | MET | VAL | LEU | VAL | GLY | ASN | LYS | CYS | ASP | LEU | ||||
| 13 | PRO | SER | ARG | THR | VAL | ASP | THR | LYS | GLN | ALA | ||||
| 14 | GLN | ASP | LEU | ALA | ARG | SER | TYR | GLY | ILE | PRO | ||||
| 15 | PHE | ILE | GLU | THR | SER | ALA | LYS | THR | ARG | GLN | ||||
| 16 | GLY | VAL | ASP | ASP | ALA | PHE | TYR | THR | LEU | VAL | ||||
| 17 | ARG | GLU | ILE | ARG | LYS | HIS | LYS | GLU | LYS | MET | ||||
| 18 | SER |
Entity 2, GNP - C10 H17 N6 O13 P3 - 522.196 Da.
| 1 | GNP |
Entity 3, Mg - Mg - 24.305 Da.
| 1 | MG |
Related Database Links:
| BMRB | 18529 25114 25115 25116 26635 |
| PDB | 2MSC 2MSD 2MSE 3GFT 4DSN 4DSO 4DST 4DSU 4EPR 4EPT 4EPV 4EPW 4EPX 4EPY 4L8G 4LDJ 4LPK 4LRW 4LUC 4LV6 4LYF 4LYH 4LYJ 4M1O 4M1S 4M1T 4M1W 4M1Y 4M21 4M22 4NMM 4OBE 4PZY 4PZZ 4Q01 4Q02 4Q03 4QL3 4TQ9 4TQA 4WA7 |
| DBJ | BAE33023 BAE37609 BAF85199 BAJ17756 |
| EMBL | CAA25624 CAA26295 CAA37336 CAA59755 CAA76678 |
| GB | AAA35689 AAA35690 AAA36554 AAA42011 AAA49429 |
| PIR | A54321 |
| PRF | 0909262B 1707302A |
| REF | NP_001003744 NP_001028153 NP_001095209 NP_001103471 NP_001184192 |
| SP | O42277 P79800 Q05147 Q07983 Q5EFX7 |
| TPG | DAA29418 |
| AlphaFold | O42277 P79800 Q05147 Q07983 Q5EFX7 |
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