BMRB Entry 26635

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26635

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Title:
Backbone 1H, and 15N Chemical Shift Assignments for human K-Ras4B-GDP
Deposition date:
2015-08-13
Original release date:
2015-09-11
Authors:
Abraham, Sherwin; Chavan, Tanmay; Banerjee, Avik
Citation:

Citation: Jang, Hyunbum; Banerjee, Avik; Chavan, Tanmay; Gaponenko, Vadim; Nussinov, Ruth. "Flexible-body motions of calmodulin and the farnesylated hypervariable region yield a high-affinity interaction enabling K-Ras4B membrane extraction"  J. Biol. Chem. 292, 12544-12559 (2017).
PubMed: 28623230

Assembly members:

Assembly members:
K-Ras4B, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET42a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K-Ras4B: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNTKSFEDIHHYREQI KRVKDSEDVPMVLVGNKCDL PSRTVDTKQAQDLARSYGIP FIETSAKTRQGVDDAFYTLV REIRKHKEKMSKDGKKKKKK SKTKCVIM

Data sets:
Data typeCount
15N chemical shifts171
1H chemical shifts171

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1human K-Ras4B-GDP1

Entities:

Entity 1, human K-Ras4B-GDP 188 residues - Formula weight is not available

1   METTHRGLUTYRLYSLEUVALVALVALGLY
2   ALAGLYGLYVALGLYLYSSERALALEUTHR
3   ILEGLNLEUILEGLNASNHISPHEVALASP
4   GLUTYRASPPROTHRILEGLUASPSERTYR
5   ARGLYSGLNVALVALILEASPGLYGLUTHR
6   CYSLEULEUASPILELEUASPTHRALAGLY
7   GLNGLUGLUTYRSERALAMETARGASPGLN
8   TYRMETARGTHRGLYGLUGLYPHELEUCYS
9   VALPHEALAILEASNASNTHRLYSSERPHE
10   GLUASPILEHISHISTYRARGGLUGLNILE
11   LYSARGVALLYSASPSERGLUASPVALPRO
12   METVALLEUVALGLYASNLYSCYSASPLEU
13   PROSERARGTHRVALASPTHRLYSGLNALA
14   GLNASPLEUALAARGSERTYRGLYILEPRO
15   PHEILEGLUTHRSERALALYSTHRARGGLN
16   GLYVALASPASPALAPHETYRTHRLEUVAL
17   ARGGLUILEARGLYSHISLYSGLULYSMET
18   SERLYSASPGLYLYSLYSLYSLYSLYSLYS
19   SERLYSTHRLYSCYSVALILEMET

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks