BMRB Entry 18529
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18529
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Citation: Vo, Uybach; Embrey, Kevin; Breeze, Alexander; Golovanov, Alexander. "1H, 13C and 15N resonance assignment for the human K-Ras at physiological pH." Biomol. NMR Assignments 7, 215-219 (2013).
PubMed: 22886485
Assembly members:
K-Ras, polymer, 169 residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET28b
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 489 |
| 15N chemical shifts | 161 |
| 1H chemical shifts | 473 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | K-Ras | 1 |
| 2 | GDP | 2 |
Entities:
Entity 1, K-Ras 169 residues - Formula weight is not available
First three residues 1-3 (GSH)represent non-native tag remaining after His-tag removal. It is not included in the sequence numbering, and amide signals from these residues were not visible in the spectra.
| 1 | GLY | SER | HIS | MET | THR | GLU | TYR | LYS | LEU | VAL | ||||
| 2 | VAL | VAL | GLY | ALA | GLY | GLY | VAL | GLY | LYS | SER | ||||
| 3 | ALA | LEU | THR | ILE | GLN | LEU | ILE | GLN | ASN | HIS | ||||
| 4 | PHE | VAL | ASP | GLU | TYR | ASP | PRO | THR | ILE | GLU | ||||
| 5 | ASP | SER | TYR | ARG | LYS | GLN | VAL | VAL | ILE | ASP | ||||
| 6 | GLY | GLU | THR | CYS | LEU | LEU | ASP | ILE | LEU | ASP | ||||
| 7 | THR | ALA | GLY | GLN | GLU | GLU | TYR | SER | ALA | MET | ||||
| 8 | ARG | ASP | GLN | TYR | MET | ARG | THR | GLY | GLU | GLY | ||||
| 9 | PHE | LEU | CYS | VAL | PHE | ALA | ILE | ASN | ASN | THR | ||||
| 10 | LYS | SER | PHE | GLU | ASP | ILE | HIS | HIS | TYR | ARG | ||||
| 11 | GLU | GLN | ILE | LYS | ARG | VAL | LYS | ASP | SER | GLU | ||||
| 12 | ASP | VAL | PRO | MET | VAL | LEU | VAL | GLY | ASN | LYS | ||||
| 13 | CYS | ASP | LEU | PRO | SER | ARG | THR | VAL | ASP | THR | ||||
| 14 | LYS | GLN | ALA | GLN | ASP | LEU | ALA | ARG | SER | TYR | ||||
| 15 | GLY | ILE | PRO | PHE | ILE | GLU | THR | SER | ALA | LYS | ||||
| 16 | THR | ARG | GLN | GLY | VAL | ASP | ASP | ALA | PHE | TYR | ||||
| 17 | THR | LEU | VAL | ARG | GLU | ILE | ARG | LYS | HIS |
Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.
| 1 | GDP |
Related Database Links:
| BMRB | 17785 25114 25115 25116 26635 |
| PDB | 2MSC 2MSD 2MSE 3GFT 4DSN 4DSO 4DST 4DSU 4EPR 4EPT 4EPV 4EPW 4EPX 4EPY 4L8G 4LDJ 4LPK 4LRW 4LUC 4LV6 4LYF 4LYH 4LYJ 4M1O 4M1S 4M1T 4M1W 4M1Y 4M21 4M22 4NMM 4OBE 4PZY 4PZZ 4Q01 4Q02 4Q03 4QL3 4TQ9 4TQA 4WA7 |
| DBJ | BAE33023 BAE37609 BAF85199 BAJ17756 |
| EMBL | CAA25624 CAA26295 CAA37336 CAA59755 CAA73253 |
| GB | AAA35689 AAA35690 AAA36554 AAA42011 AAA49189 |
| PIR | A54321 |
| PRF | 0909262A 0909262B 2105181A |
| REF | NP_001003744 NP_001028153 NP_001084379 NP_001095209 NP_001103471 |
| SP | O42277 P01116 P05774 P08644 P32883 |
| TPG | DAA29418 |
| AlphaFold | O42277 P01116 P05774 P08644 P32883 |
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