BMRB Entry 17716

Name: Structure of Cu(I)Cu(II)-CopK from Cupriavidus metallidurans CH34
Published: 2011-10-12

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PDB ID: 2lel
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17716
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Cu(I)Cu(II)-CopK from Cupriavidus metallidurans CH34

Deposition date:

2011-06-16

Original release date:

2011-10-12

Authors:

Hinds, Mark; Xiao, Zhiguang; Chong, Lee Xin; Wedd, Anthony

Citation:

Citation: Ash, Miriam-Rose; Chong, Lee Xin; Maher, Megan; Hinds, Mark; Xiao, Zhiguang; Wedd, Anthony. "Molecular basis of the cooperative binding of Cu(I) and Cu(II) to the CopK protein from Cupriavidus metallidurans CH34." Biochemistry 50, 9237-9247 (2011).
PubMed: 21936507

Assembly members:

Assembly members:
Cu(I)Cu(II)-CopK, polymer, 74 residues, 8294.617 Da.
CU1, non-polymer, 63.546 Da.
CU, non-polymer, 63.546 Da.
HOH, non-polymer, 18.015 Da.

Natural source:

Natural source: Common Name: Cupriavidus metallidurans Taxonomy ID: 266264 Superkingdom: Bacteria Kingdom: not available Genus/species: Cupriavidus metallidurans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCX07

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cu(I)Cu(II)-CopK: VDMSNVVKTYDLQDGSKVHV FKDGKMGMENKFGKSMNMPE GKVMETRDGTKIIMKGNEIF RLDEALRKGHSEGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	252
15N chemical shifts	68
1H chemical shifts	400

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cu(I)Cu(II)-CopK	1
2	COPPER (I) ION	2
3	CU2	3
4	water	4

Entities:

Entity 1, Cu(I)Cu(II)-CopK 74 residues - 8294.617 Da.

1-74 represent the processed form of the protein found in the periplasim

1

VAL

ASP

MET

SER

ASN

VAL

LYS

THR

TYR

2

ASP

LEU

GLN

ASP

GLY

SER

LYS

VAL

HIS

VAL

3

PHE

LYS

ASP

GLY

LYS

MET

GLY

MET

GLU

ASN

4

LYS

PHE

GLY

LYS

SER

MET

ASN

MET

PRO

GLU

5

GLY

LYS

VAL

MET

GLU

THR

ARG

ASP

GLY

THR

6

LYS

ILE

MET

LYS

GLY

ASN

GLU

ILE

PHE

7

ARG

LEU

ASP

GLU

ALA

LEU

ARG

LYS

GLY

HIS

8

SER

GLU

GLY

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1

CU1

Entity 3, CU2 - Cu - 63.546 Da.

1

CU

Entity 4, water - H2 O - 18.015 Da.

1

HOH

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1

BMRB	15655 16408
PDB	2K0Q 2KM0 2LEL 3DSO 3DSP 3N7D 3N7E
EMBL	CAI11334
GB	ABF12967 EKZ95342
REF	WP_008652571 YP_145685
SP	Q58AD3
AlphaFold	Q58AD3

BMRB Entry 17716

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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