BMRB Entry 17627

Name: 1H, 13C, and 15N chemical shift assignments for apo actinin-2 C-terminal EF-hand (Act2-EF34)
Published: 2011-06-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17627

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for apo actinin-2 C-terminal EF-hand (Act2-EF34)

Deposition date:

2011-05-05

Original release date:

2011-06-03

Authors:

Au, Yunghan; Beck, Moriah; Campbell, Sharon; Pastore, Annalisa

Citation:

Citation: Au, Yunghan; Pastore, Annalisa. "The Interaction of alpha-Actinin-2 with ZASP and Titin" (2004).

Assembly members:

Assembly members:
Act2-EF34, polymer, 75 residues, 8071.05 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Act2-EF34: GAMADTDTAEQVIASFRILA SDKPYILAEELRRELPPDQA QYCIKRMPAYSGPGSVPGAL DYAAFSSALYGESDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	135
15N chemical shifts	67
1H chemical shifts	276

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ef-Hands 3,4 From Alpha-Actinin	1

Entities:

Entity 1, Ef-Hands 3,4 From Alpha-Actinin 75 residues - 8071.05 Da.

N-terminal residue of natural sequence replaced by M N-terminal GA left after cleavage from fusion protein

1

GLY

ALA

MET

ALA

ASP

THR

ASP

THR

ALA

GLU

2

GLN

VAL

ILE

ALA

SER

PHE

ARG

ILE

LEU

ALA

3

SER

ASP

LYS

PRO

TYR

ILE

LEU

ALA

GLU

4

LEU

ARG

GLU

LEU

PRO

ASP

GLN

ALA

5

GLN

TYR

CYS

ILE

LYS

ARG

MET

PRO

ALA

TYR

6

SER

GLY

PRO

GLY

SER

VAL

PRO

GLY

ALA

LEU

7

ASP

TYR

ALA

PHE

SER

ALA

LEU

TYR

8

GLY

GLU

SER

ASP

LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1

BMRB	17626 4453 4454
PDB	1H8B 4D1E
DBJ	BAB22865 BAD92758 BAG37672 BAH11921 BAH12587
EMBL	CAB61269
GB	AAA51583 AAF76325 AAH47901 AAH51770 AAH89579
REF	NP_001029807 NP_001094 NP_001163796 NP_001230595 NP_001265272
SP	P35609 Q3ZC55 Q9JI91
AlphaFold	P35609 Q3ZC55 Q9JI91

BMRB Entry 17627

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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