BMRB Entry 4453

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4453

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Title:
Assignment of the 1H, 13C and 15N Resonances of the C-terminal EF-Hands of Alpha Actinin in a 14 kDa Complex with Z-Repeat 7 of Titin
Deposition date:
1999-11-10
Original release date:
2000-03-22
Authors:
Atkinson, R.; Joseph, Catherine; Kelly, Geoff; Muskett, Frederick; Frenkiel, Thomas; Pastore, Annalisa
Citation:

Citation: Atkinson, R.; Joseph, Catherine; Kelly, Geoff; Muskett, Frederick; Frenkiel, Thomas; Pastore, Annalisa. "Letter to the Editor: Assignment of the 1H, 13C and 15N resonances of the C-terminal EF-hands of alpha-actinin in a 14 kDa complex with Z-repeat 7 of titin"  J. Biomol. NMR 16, 277-278 (2000).

Assembly members:

Assembly members:
alpha-actinin C-terminal EF-hands, polymer, 75 residues, 8071.05 Da.
titin Z-repeat 7, polymer, 53 residues, 5659.33 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha-actinin C-terminal EF-hands: GAMADTDTAEQVIASFRILA SDKPYILAEELRRELPPDQA QYCIKRMPAYSGPGSVPGAL DYAAFSSALYGESDL
titin Z-repeat 7: GAMGKVGVGKKAEAVATVVA AVDQARVREPREPGLPEDSY AQQTTLEYGYKEH

Data sets:
Data typeCount
1H chemical shifts455
13C chemical shifts291
15N chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-actininC-terminal EF-hands1
2titin Z-repeat 72

Entities:

Entity 1, alpha-actininC-terminal EF-hands 75 residues - 8071.05 Da.

1   GLYALAMETALAASPTHRASPTHRALAGLU
2   GLNVALILEALASERPHEARGILELEUALA
3   SERASPLYSPROTYRILELEUALAGLUGLU
4   LEUARGARGGLULEUPROPROASPGLNALA
5   GLNTYRCYSILELYSARGMETPROALATYR
6   SERGLYPROGLYSERVALPROGLYALALEU
7   ASPTYRALAALAPHESERSERALALEUTYR
8   GLYGLUSERASPLEU

Entity 2, titin Z-repeat 7 53 residues - 5659.33 Da.

1   GLYALAMETGLYLYSVALGLYVALGLYLYS
2   LYSALAGLUALAVALALATHRVALVALALA
3   ALAVALASPGLNALAARGVALARGGLUPRO
4   ARGGLUPROGLYLEUPROGLUASPSERTYR
5   ALAGLNGLNTHRTHRLEUGLUTYRGLYTYR
6   LYSGLUHIS

Samples:

sample_one: alpha-actinin C-terminal EF-hands, [U-15N], 0.5 – 1.0 mM; titin Z-repeat 70.5 – 1.0 mM; sodium phosphate 20 mM

sample_two: alpha-actinin C-terminal EF-hands, [U-13C; U-15N], 0.5 – 1.0 mM; titin Z-repeat 70.5 – 1.0 mM; sodium phosphate 20 mM

sample_conditions: pH: 6.6 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablesample_conditions
2D 1H-13C HSQCnot availablenot availablesample_conditions
3D HNCAnot availablenot availablesample_conditions
3D HN(CO)CAnot availablenot availablesample_conditions
3D HNCOnot availablenot availablesample_conditions
3D CBCANHnot availablenot availablesample_conditions
3D CBCA(CO)NHnot availablenot availablesample_conditions
3D HCCH-TOCSYnot availablenot availablesample_conditions
3D 1H-15N NOESY-HSQCnot availablenot availablesample_conditions
3D 1H-15N TOCSY-HSQCnot availablenot availablesample_conditions
3D 1H-13C NOESY-HSQCnot availablenot availablesample_conditions
3D HNHAnot availablenot availablesample_conditions
3D HNHBnot availablenot availablesample_conditions

Software:

nmrPipe - processing

XEASY - analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

BMRB 17626 17627 4454 4454
PDB
DBJ BAB22865 BAD92758 BAG37672 BAH11921 BAH12587
EMBL CAB61269 CAA77028
GB AAA51583 AAF76325 AAH47901 AAH51770 AAH89579
REF NP_001029807 NP_001094 NP_001163796 NP_001230595 NP_001265272
SP P35609 Q3ZC55 Q9JI91
AlphaFold P35609 Q3ZC55 Q9JI91

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks