BMRB Entry 4454
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4454
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Atkinson, R.; Joseph, Catherine; Dal Piaz, Fabrizio; Birolo, Leyla; Stier, Gunter; Pucci, Piero; Pastore, Annalisa. "The Binding of Alpha-actinin to Titin: Implications for Z-disk Assembly" Biochemistry ., .-..
Assembly members:
alpha-actinin C-terminal EF-hands, polymer, 75 residues, 8071.05 Da.
titin Z-repeat 7, polymer, 51 residues, 5659.33 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
alpha-actinin C-terminal EF-hands: GAMADTDTAEQVIASFRILA
SDKPYILAEELRRELPPDQA
QYCIKRMPAYSGPGSVPGAL
DYAAFSSALYGESDL
titin Z-repeat 7: MGKVGVGKKAEAVATVVAAV
DQARVREPREPGLPEDSYAQ
QTTLEYGYKEH
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 174 |
| 15N chemical shifts | 31 |
| coupling constants | 31 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alpha-actinin_C-terminal EF-hands | 1 |
| 2 | titin Z-repeat 7 | 2 |
Entities:
Entity 1, alpha-actinin_C-terminal EF-hands 75 residues - 8071.05 Da.
| 1 | GLY | ALA | MET | ALA | ASP | THR | ASP | THR | ALA | GLU | ||||
| 2 | GLN | VAL | ILE | ALA | SER | PHE | ARG | ILE | LEU | ALA | ||||
| 3 | SER | ASP | LYS | PRO | TYR | ILE | LEU | ALA | GLU | GLU | ||||
| 4 | LEU | ARG | ARG | GLU | LEU | PRO | PRO | ASP | GLN | ALA | ||||
| 5 | GLN | TYR | CYS | ILE | LYS | ARG | MET | PRO | ALA | TYR | ||||
| 6 | SER | GLY | PRO | GLY | SER | VAL | PRO | GLY | ALA | LEU | ||||
| 7 | ASP | TYR | ALA | ALA | PHE | SER | SER | ALA | LEU | TYR | ||||
| 8 | GLY | GLU | SER | ASP | LEU |
Entity 2, titin Z-repeat 7 51 residues - 5659.33 Da.
| 1 | MET | GLY | LYS | VAL | GLY | VAL | GLY | LYS | LYS | ALA | ||||
| 2 | GLU | ALA | VAL | ALA | THR | VAL | VAL | ALA | ALA | VAL | ||||
| 3 | ASP | GLN | ALA | ARG | VAL | ARG | GLU | PRO | ARG | GLU | ||||
| 4 | PRO | GLY | LEU | PRO | GLU | ASP | SER | TYR | ALA | GLN | ||||
| 5 | GLN | THR | THR | LEU | GLU | TYR | GLY | TYR | LYS | GLU | ||||
| 6 | HIS |
Samples:
sample_one: alpha-actinin C-terminal EF-hands 0.7 mM; titin Z-repeat 7, [U-15N], 0.7 mM; sodium phosphate 20.0 mM
sample_conditions: pH: 6.6 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_one | not available | sample_conditions |
| 2D TOCSY | sample_one | not available | sample_conditions |
| 2D 1H-15N HSQC | sample_one | not available | sample_conditions |
| 3D 15N NOESY-HSQC | sample_one | not available | sample_conditions |
| 3D 15N TOCSY-HSQC | sample_one | not available | sample_conditions |
| 3D HNHA | sample_one | not available | sample_conditions |
| 3D HNHB | sample_one | not available | sample_conditions |
Software:
nmrPipe - processing
XEASY - analysis
NMR spectrometers:
- Varian UnityPlus 500 MHz
- Varian UnityPlus 600 MHz
Related Database Links:
| BMRB | 17626 17627 4453 4453 |
| PDB | |
| DBJ | BAB22865 BAD92758 BAG37672 BAH11921 BAH12587 |
| EMBL | CAB61269 CAA77028 |
| GB | AAA51583 AAF76325 AAH47901 AAH51770 AAH89579 |
| REF | NP_001029807 NP_001094 NP_001163796 NP_001230595 NP_001265272 |
| SP | P35609 Q3ZC55 Q9JI91 |
| AlphaFold | P35609 Q3ZC55 Q9JI91 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks