BMRB Entry 17238
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PDB ID: 2l4e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17238
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Burschowsky, Daniel; Rudolf, Fabian; Mattle, Daniel; Peter, Matthias; Wider, Gerhard. "Structural analysis of the ubiquitin-associated domain (UBA) of yeast Dcn1 in complex with ubiquitin (WORKING TITLE)" .
Assembly members:
Dcn1-UBA, polymer, 59 residues, 6914.6 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T
Entity Sequences (FASTA):
Dcn1-UBA: GSIKRKDASPEQEAIESFTS
LTKCDPKVSRKYLQRNHWNI
NYALNDYYDKEIGTFTDEV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 200 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 417 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dcn1-UBA | 1 |
Entities:
Entity 1, Dcn1-UBA 59 residues - 6914.6 Da.
N-terminal residues number 4 and 5 (GS) are non-native and leftovers from thrombin cleavage
| 1 | GLY | SER | ILE | LYS | ARG | LYS | ASP | ALA | SER | PRO | ||||
| 2 | GLU | GLN | GLU | ALA | ILE | GLU | SER | PHE | THR | SER | ||||
| 3 | LEU | THR | LYS | CYS | ASP | PRO | LYS | VAL | SER | ARG | ||||
| 4 | LYS | TYR | LEU | GLN | ARG | ASN | HIS | TRP | ASN | ILE | ||||
| 5 | ASN | TYR | ALA | LEU | ASN | ASP | TYR | TYR | ASP | LYS | ||||
| 6 | GLU | ILE | GLY | THR | PHE | THR | ASP | GLU | VAL |
Related Database Links:
| BMRB | 17239 |
| PDB | 2L4E 2L4F 3BQ3 |
| DBJ | GAA25010 |
| EMBL | CAA61706 CAA62639 CAA97697 CAY81363 |
| GB | AAB82374 AHY78522 AJP40299 AJV46201 AJV46655 |
| REF | NP_013229 |
| SP | Q12395 |
| TPG | DAA09439 |
| AlphaFold | Q12395 |
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