BMRB Entry 17138

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PDB ID: 2l2l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17138
MolProbity Validation Chart

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Title:
Solution structure of the coiled-coil complex between MBD2 and p66alpha
Deposition date:
2010-08-20
Original release date:
2012-08-02
Authors:
Williams, David; Scarsdale, Joe
Citation:

Citation: Gnanapragasam, Merlin; Scarsdale, Joe; Amaya, Maria; Webb, Heather; Walavalkar, Ninad; Ginder, Gordon; David, Williams. "Solution structure and functional analyses of a coiled-coil interaction between MBD2 and p66alpha integral to the formation of the MeCP1 nucleosome remodeling complex"  .

Assembly members:

Assembly members:
p66-alpha, polymer, 43 residues, 5116.060 Da.
MBD2, polymer, 36 residues, 4181.826 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p66-alpha: GSPEERERMIKQLKEELRLE EAKLVLLKKLRQSQIQKEAT AQK
MBD2: GSKAFIVTDEDIRKQEERVQ QVRKKLEEALMADILS

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts74
1H chemical shifts588

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1p66-alpha1
2MBD22

Entities:

Entity 1, p66-alpha 43 residues - 5116.060 Da.

N-terminal Gly-Ser residues represent non-native sequence from an affinity tag.

1   GLYSERPROGLUGLUARGGLUARGMETILE
2   LYSGLNLEULYSGLUGLULEUARGLEUGLU
3   GLUALALYSLEUVALLEULEULYSLYSLEU
4   ARGGLNSERGLNILEGLNLYSGLUALATHR
5   ALAGLNLYS

Entity 2, MBD2 36 residues - 4181.826 Da.

N-terminal Gly-Ser residues represent non-native sequence from an affinity tag. Numbering reflects the MBD2b isoform.

1   GLYSERLYSALAPHEILEVALTHRASPGLU
2   ASPILEARGLYSGLNGLUGLUARGVALGLN
3   GLNVALARGLYSLYSLEUGLUGLUALALEU
4   METALAASPILELEUSER

Related Database Links:

PDB 2L2L
DBJ BAC40736 BAE89133 BAG59359 BAG73190
EMBL CAH65456 CAH10725
GB AAH11684 AAH38221 AAH89902 AAO31797 ABZ92025 AAC18050 AAC68871 AAC68872 AAD50372 AAD56597
REF NP_001012570 NP_001013903 NP_001106817 NP_001273379 NP_001287875 NP_001108497 NP_003918 NP_034903 XP_001082249 XP_001362190
SP Q86YP4 Q8CHY6 Q9UBB5 Q9Z2E1
TPG DAA15869
AlphaFold Q86YP4 Q8CHY6 Q9UBB5 Q9Z2E1

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