BMRB Entry 17026

Title:
Solution NMR Structure of protein BT2368 from Bacteroides thetaiotaomicron, Northeast Structural Genomics Consortium Target BtR375
Deposition date:
2010-06-29
Original release date:
2010-08-23
Authors:
Eletsky, Alexander; Lee, Hsiau-Wei; Wang, Dongyan; Ciccosanti, Colleen; Hamilton, Keith; Acton, Thomas; Xiao, Rong; Everett, John; Prestegard, James; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Eletsky, Alexander; Lee, Hsiau-Wei; Wang, Dongyan; Ciccosanti, Colleen; Hamilton, Keith; Acton, Thomas; Xiao, Rong; Everett, John; Prestegard, James; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR Structure of protein BT2368 from Bacteroides thetaiotaomicron"  To be published ., .-..

Assembly members:

Assembly members:
BtR375, polymer, 82 residues, 9220.528 Da.

Natural source:

Natural source:   Common Name: B. thetaiotaomicron   Taxonomy ID: 818   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacteroides thetaiotaomicron

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 21-23C

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts91
1H chemical shifts554
residual dipolar couplings94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BtR375_11
2BtR375_21

Entities:

Entity 1, BtR375_1 82 residues - 9220.528 Da.

Residues 75-82 represent a non-native affinity tag

1   METASPLYSLYSILEVALGLYALAASNALA
2   GLYLYSVALTRPHISALALEUASNGLUALA
3   ASPGLYILESERILEPROGLULEUALAARG
4   LYSVALASNLEUSERVALGLUSERTHRALA
5   LEUALAVALGLYTRPLEUALAARGGLUASN
6   LYSVALVALILEGLUARGLYSASNGLYLEU
7   ILEGLUILETYRASNGLUGLYHISPHEASP
8   PHESERPHEGLYLEUGLUHISHISHISHIS
9   HISHIS

Related Database Links:

PDB 2L02
EMBL CDE77590 CUN18104 CUQ04359 CUQ17116
GB AAO77475 ALJ40308 EES66434 EFI02088 EOS01101
REF NP_811281 WP_008763800

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks