BMRB Entry 16899

Assembly:

Entities:

Entity 1, UBLCP1 81 residues - 9015.780 Da.

this protein is the mutant of UBL domain of UBLCP1. The mutation site is M5. Original sequence is "MALPIIVK" nad mutant sequence is "MALPMIVK".

Samples:

sample_1: UBLI5M, [U-99% 15N], 1.5 mM; H2O 90%; D2O 10%; HEPES 10 mM; NaCl 100 mM; DTT 5 mM

sample_2: UBLI5M, [U-99% 13C; U-99% 15N], 1.5 mM; H2O 90%; D2O 10%; HEPES 10 mM; NaCl 100 mM; DTT 5 mM

sample_3: UBLI5M, [U-100% 13C], 1.5 mM; D2O 100%; HEPES 10 mM; NaCl 100 mM; DTT 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CYANA v2.2.5, P.GUNTERT ET AL. - refinement, structure solution

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - processing

NMR spectrometers:

• Bruker DRX 500 MHz
• Bruker DRX 900 MHz