BMRB Entry 16788
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PDB ID: 2kvq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16788
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Burmann, Bjoern; Schweimer, Kristian; Luo, Xiao; Wahl, Markus; Stitt, Barbara; Gottesman, Max; Roesch, Paul. "A NusE:NusG Complex Links Transcription and Translation" Science 328, 501-504 (2010).
PubMed: 20413501
Assembly members:
NusE, polymer, 87 residues, 8937.450 Da.
NusG-CTD, polymer, 63 residues, 6639.524 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P
Entity Sequences (FASTA):
NusE: GPLGSMQNQRIRIRLKAFDH
RLIDQATAEIVETAKRTGAQ
VRGPIPLPTRSRTHLRLVDI
VEPTEKTVDALMRLDLAAGV
DVQISLG
NusG-CTD: GAMGRPKTLFEPGEMVRVND
GPFADFNGVVEEVDYEKSRL
KVSVSIFGRATPVELDFSQV
EKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 410 |
| 15N chemical shifts | 120 |
| 1H chemical shifts | 928 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NusE | 1 |
| 2 | NusG | 2 |
Entities:
Entity 1, NusE 87 residues - 8937.450 Da.
| 1 | GLY | PRO | LEU | GLY | SER | MET | GLN | ASN | GLN | ARG | ||||
| 2 | ILE | ARG | ILE | ARG | LEU | LYS | ALA | PHE | ASP | HIS | ||||
| 3 | ARG | LEU | ILE | ASP | GLN | ALA | THR | ALA | GLU | ILE | ||||
| 4 | VAL | GLU | THR | ALA | LYS | ARG | THR | GLY | ALA | GLN | ||||
| 5 | VAL | ARG | GLY | PRO | ILE | PRO | LEU | PRO | THR | ARG | ||||
| 6 | SER | ARG | THR | HIS | LEU | ARG | LEU | VAL | ASP | ILE | ||||
| 7 | VAL | GLU | PRO | THR | GLU | LYS | THR | VAL | ASP | ALA | ||||
| 8 | LEU | MET | ARG | LEU | ASP | LEU | ALA | ALA | GLY | VAL | ||||
| 9 | ASP | VAL | GLN | ILE | SER | LEU | GLY |
Entity 2, NusG 63 residues - 6639.524 Da.
| 1 | GLY | ALA | MET | GLY | ARG | PRO | LYS | THR | LEU | PHE | ||||
| 2 | GLU | PRO | GLY | GLU | MET | VAL | ARG | VAL | ASN | ASP | ||||
| 3 | GLY | PRO | PHE | ALA | ASP | PHE | ASN | GLY | VAL | VAL | ||||
| 4 | GLU | GLU | VAL | ASP | TYR | GLU | LYS | SER | ARG | LEU | ||||
| 5 | LYS | VAL | SER | VAL | SER | ILE | PHE | GLY | ARG | ALA | ||||
| 6 | THR | PRO | VAL | GLU | LEU | ASP | PHE | SER | GLN | VAL | ||||
| 7 | GLU | LYS | ALA |
Related Database Links:
| PDB | 2KVQ 3D3B 3D3C 3IMQ 2JVV |
| EMBL | CDK48609 CDK60294 CDL02401 CDL28926 CDL47417 CAD09492 CAP78438 CAQ34328 CAQ91223 CAR00955 |
| GB | ESD90132 AAA24622 AAC43080 AAC76956 AAF33495 AAG59178 |
| BMRB | 15490 |
| DBJ | BAB38328 BAE77338 BAG79793 BAH61119 BAI28242 |
| PIR | AB0934 |
| REF | NP_290613 NP_312932 NP_418409 NP_457922 NP_463017 |
| SP | P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1 |
| AlphaFold | P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1 |
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