BMRB Entry 16686

Click here to enlarge.

PDB ID: 2kt7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16686
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution NMR structure of mucin-binding domain of protein lmo0835 from Listeria monocytogenes. Northeast Structural Genomics Consortium Target LmR64A
Deposition date:
2010-01-20
Original release date:
2010-02-25
Authors:
Eletsky, Alexander; He, Yunfen; Lee, Dan; Ciccosanti, Colleen; Janjua, Haleema; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Eletsky, Alexander; He, Yunfen; Lee, Dan; Ciccosanti, Colleen; Janjua, Haleema; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR structure of mucin-binding domain of protein lmo0835 from Listeria monocytogenes."  Proteins: Struct. Funct. Genet. ., .-..

Assembly members:

Assembly members:
lmr64a_protein, polymer, 102 residues, 11501.830 Da.

Natural source:

Natural source:   Common Name: Listeria monocytogenes   Taxonomy ID: 1639   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Listeria monocytogenes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lmr64a_protein: MDTNNFTVKVEYVDADGAEI APSDTLTDYHYVSTPKDIPG YKLREIPHNATGNITDTGII VRYIYDKIIDVSYVDETGKD LLPVVEIINSEAAVLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts101
1H chemical shifts689

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lmr64a_protein1

Entities:

Entity 1, lmr64a_protein 102 residues - 11501.830 Da.

Residues 35-127 correspond to the fragment 34-126 of the published native sequence, with the exception of strain variations I58T, T101I and R105S. Residues 128-133 represent a non-native affinity tag, and methionine at position 34 was introduced to promote expression.

1   METASPTHRASNASNPHETHRVALLYSVAL
2   GLUTYRVALASPALAASPGLYALAGLUILE
3   ALAPROSERASPTHRLEUTHRASPTYRHIS
4   TYRVALSERTHRPROLYSASPILEPROGLY
5   TYRLYSLEUARGGLUILEPROHISASNALA
6   THRGLYASNILETHRASPTHRGLYILEILE
7   VALARGTYRILETYRASPLYSILEILEASP
8   VALSERTYRVALASPGLUTHRGLYLYSASP
9   LEULEUPROVALVALGLUILEILEASNSER
10   GLUALAALAVALLEUGLUHISHISHISHIS
11   HISHIS

Related Database Links:

PDB 2KT7
DBJ GAM91864 GAM94621
EMBL CAS04619 CBY03397 CBY48425 CBY66913 CBY69752
GB AAT03632 ABN80104 AFH79417 AGR06974 AGR12775
REF WP_003733401 WP_003743756 WP_010958814 WP_012681181 WP_021496215

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks