BMRB Entry 15948

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PDB ID: 2kiz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15948
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for the RING finger of the E3 ubiquitin ligase Arkadia (RNF111)
Deposition date:
2008-09-10
Original release date:
2009-01-16
Authors:
Kandias, Nikolaos; Chasapis, Christos; Episkopou, Vasso; Bentrop, Detlef; Spyroulias, Georgios
Citation:

Citation: Kandias, Nikolaos; Chasapis, Christos; Bentrop, Detlef; Episkopou, Vasso; Spyroulias, Georgios. "High yield expression and NMR characterization of Arkadia E3 ubiquitin ligase RING-H2 finger domain"  Biochem. Biophys. Res. Commun. 378, 498-502 (2009).
PubMed: 19032943

Assembly members:

Assembly members:
E3_ubiquitin_ligase_Arkadia, polymer, 69 residues, 7951.0 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex-4T.1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E3_ubiquitin_ligase_Arkadia: MKQDGEEGTEEDTEEKCTIC LSILEEGEDVRRLPCMHLFH QVCVDQWLITNKKCPICRVD IEAQLPSES

Data sets:
Data typeCount
13C chemical shifts290
15N chemical shifts71
1H chemical shifts467

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RING Finger1
2Zinc ion, 12
3Zinc ion, 22

Entities:

Entity 1, RING Finger 69 residues - 7951.0 Da.

1   METLYSGLNASPGLYGLUGLUGLYTHRGLU
2   GLUASPTHRGLUGLULYSCYSTHRILECYS
3   LEUSERILELEUGLUGLUGLYGLUASPVAL
4   ARGARGLEUPROCYSMETHISLEUPHEHIS
5   GLNVALCYSVALASPGLNTRPLEUILETHR
6   ASNLYSLYSCYSPROILECYSARGVALASP
7   ILEGLUALAGLNLEUPROSERGLUSER

Entity 2, Zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2KIZ
DBJ BAC04531 BAC29394 BAC33245 BAD18471 BAD18633
EMBL CAB75669 CAD98031 CAH93440
GB AAH10369 AAH20984 AAH54842 AAH60862 AAH69835
REF NP_001095709 NP_001100306 NP_001124565 NP_001186680 NP_001257457
SP Q5R476 Q6ZNA4 Q99ML9
TPG DAA25343
AlphaFold Q5R476 Q6ZNA4 Q99ML9

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