BMRB Entry 15747

Title:
Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger
Deposition date:
2008-05-01
Original release date:
2008-05-29
Authors:
Reddy, Tyler; Ding, J.; Li, X.; Sykes, Brian; Fliegel, Larry; Rainey, Jan
Citation:

Citation: Reddy, Tyler; Ding, J.; Li, X.; Sykes, Brian; Rainey, Jan; Fliegel, Larry. "Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger"  J. Biol. Chem. 283, 22018-22030 (2008).
PubMed: 18508767

Assembly members:

Assembly members:
TMIX_peptide, polymer, 33 residues, 3444.226 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TMIX_peptide: XKSYMAYLSAELFHLSGIMA LIASGVVMRPKKX

Data sets:
Data typeCount
1H chemical shifts235
13C chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TMIX_peptide1

Entities:

Entity 1, TMIX_peptide 33 residues - 3444.226 Da.

1   ACELYSSERTYRMETALATYRLEUSERALA
2   GLULEUPHEHISLEUSERGLYILEMETALA
3   LEUILEALASERGLYVALVALMETARGPRO
4   LYSLYSNH2

Samples:

sample_TMIX_peptide: TMIX peptide 0.9 ± 0.1 mM; DPC-d38 75 mM; DSS 0.25 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 303.15 K; pH: 5.05; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_TMIX_peptideisotropicsample_conditions_1
2D 1H-1H TOCSYsample_TMIX_peptideisotropicsample_conditions_1
2D 1H-1H NOESYsample_TMIX_peptideisotropicsample_conditions_1

Software:

X-PLOR_NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY v3.112, Goddard - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE29332 BAE32882 BAE38221 BAG36869 BAG62158
EMBL CAA42558 CAA43721 CAA48771 CAH23227
GB AAA31092 AAA91483 AAA92976 AAA98479 AAB20633
REF NP_001007104 NP_001095191 NP_001233682 NP_001273957 NP_003038
SP P19634 P23791 P26431 P48761 P48762
TPG DAA32066
AlphaFold P48761 P19634 P23791 P26431 P48762